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Radioimmunoassay for detection of antibodies to Neisseria gonorrhoeae.
J Clin Microbiol. 1982 Jun; 15(6):1001-8.JC

Abstract

A radioimmunoassay has been developed and evaluated for the serological diagnosis of gonorrhea. Purified gonococcal antigen was obtained from a culture of Neisseria gonorrhoeae (B370) and labeled with 125I for use in a double-antibody test system. The test was evaluated in populations segregated by sex and risk. The specificity of the assay in females was 90.2% (55/61) in low risk, 82.2% (2,245/ 2,732) in medium risk, and 54.1% (335/619) in high risk. The sensitivity was 69% (20/29) in medium risk and 78.3% (288/367) in high risk. In males, test specificity was 92.3% (24/26) in low risk and 50% (48/96) in high risk. The sensitivity was 70.8% (143/202) in the high-risk group. The data in this study indicate that this assay should not be employed for screening of either high- or medium-risk populations.

Authors

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Pub Type(s)

Journal Article

Language

eng

PubMed ID

6809784

Citation

Usategui, M, et al. "Radioimmunoassay for Detection of Antibodies to Neisseria Gonorrhoeae." Journal of Clinical Microbiology, vol. 15, no. 6, 1982, pp. 1001-8.
Usategui M, Savard EV, Mondabaugh SM, et al. Radioimmunoassay for detection of antibodies to Neisseria gonorrhoeae. J Clin Microbiol. 1982;15(6):1001-8.
Usategui, M., Savard, E. V., Mondabaugh, S. M., & Keigher, N. L. (1982). Radioimmunoassay for detection of antibodies to Neisseria gonorrhoeae. Journal of Clinical Microbiology, 15(6), 1001-8.
Usategui M, et al. Radioimmunoassay for Detection of Antibodies to Neisseria Gonorrhoeae. J Clin Microbiol. 1982;15(6):1001-8. PubMed PMID: 6809784.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Radioimmunoassay for detection of antibodies to Neisseria gonorrhoeae. AU - Usategui,M, AU - Savard,E V, AU - Mondabaugh,S M, AU - Keigher,N L, PY - 1982/6/1/pubmed PY - 1982/6/1/medline PY - 1982/6/1/entrez SP - 1001 EP - 8 JF - Journal of clinical microbiology JO - J. Clin. Microbiol. VL - 15 IS - 6 N2 - A radioimmunoassay has been developed and evaluated for the serological diagnosis of gonorrhea. Purified gonococcal antigen was obtained from a culture of Neisseria gonorrhoeae (B370) and labeled with 125I for use in a double-antibody test system. The test was evaluated in populations segregated by sex and risk. The specificity of the assay in females was 90.2% (55/61) in low risk, 82.2% (2,245/ 2,732) in medium risk, and 54.1% (335/619) in high risk. The sensitivity was 69% (20/29) in medium risk and 78.3% (288/367) in high risk. In males, test specificity was 92.3% (24/26) in low risk and 50% (48/96) in high risk. The sensitivity was 70.8% (143/202) in the high-risk group. The data in this study indicate that this assay should not be employed for screening of either high- or medium-risk populations. SN - 0095-1137 UR - https://www.unboundmedicine.com/medline/citation/6809784/Radioimmunoassay_for_detection_of_antibodies_to_Neisseria_gonorrhoeae_ L2 - http://jcm.asm.org/cgi/pmidlookup?view=long&pmid=6809784 DB - PRIME DP - Unbound Medicine ER -