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Release of slow-reacting substance of anaphylaxis from dispersed pig lung cells: effect of cyclo-oxygenase and lipoxygenase inhibitors.
J Allergy Clin Immunol. 1981 Jun; 67(6):426-34.JA

Abstract

Study of the release of slow-reacting substance of anaphylaxis (SRS-A) from lung cells has been hampered by the lack of suitable animal model. Using both immunologic and pharmacologic stimuli, we have obtained histamine and SRS-A release from dispersed pig lung cells containing 6% mast cells (with a histamine content of 1.9 pg/cell). Lung cells dispersed from actively sensitized (with intratracheal Ascaris antigen) but not unsensitized pigs released both histamine (mean net release 33%) and SRS-A (mean release, 47 units/10(7) cells) when challenged with Ascaris antigen. Greater release of histamine (mean net release 52%) and of slow-reacting substance (SRS) (mean release 701 units/10(7) cells) was induced by challenge with the calcium ionophore A23187. The pharmacologic and physicochemical characteristics of the SRS together with its profile of enzymatic inactivation resembled those described for SRA-A released from human lung. Both antigen-induced and A23187-induced SRS(-A) release were enhanced by indomethacin, a cyclo-oxygenase inhibitor, but inhibited by both phenidone (IC50 35 microM) and eicosatetraenoic acid (IC50 15 microM), inhibitors of both cyclo-oxygenase and lipoxygenase, confirming that generation of SRS(-A) by either stimulus required an intact lipoxygenase pathway of arachidonic acid metabolism.

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Publisher Full Text

Authors

Paterson NA
No affiliation info available
Burka JF
No affiliation info available
Craig ID
No affiliation info available

MeSH

AnimalsAntigensArachidonic AcidArachidonic AcidsCalcimycinLungSRS-ASwine

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

6809801

Citation

Paterson, N A., et al. "Release of Slow-reacting Substance of Anaphylaxis From Dispersed Pig Lung Cells: Effect of Cyclo-oxygenase and Lipoxygenase Inhibitors." The Journal of Allergy and Clinical Immunology, vol. 67, no. 6, 1981, pp. 426-34.
Paterson NA, Burka JF, Craig ID. Release of slow-reacting substance of anaphylaxis from dispersed pig lung cells: effect of cyclo-oxygenase and lipoxygenase inhibitors. J Allergy Clin Immunol. 1981;67(6):426-34.
Paterson, N. A., Burka, J. F., & Craig, I. D. (1981). Release of slow-reacting substance of anaphylaxis from dispersed pig lung cells: effect of cyclo-oxygenase and lipoxygenase inhibitors. The Journal of Allergy and Clinical Immunology, 67(6), 426-34.
Paterson NA, Burka JF, Craig ID. Release of Slow-reacting Substance of Anaphylaxis From Dispersed Pig Lung Cells: Effect of Cyclo-oxygenase and Lipoxygenase Inhibitors. J Allergy Clin Immunol. 1981;67(6):426-34. PubMed PMID: 6809801.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Release of slow-reacting substance of anaphylaxis from dispersed pig lung cells: effect of cyclo-oxygenase and lipoxygenase inhibitors. AU - Paterson,N A, AU - Burka,J F, AU - Craig,I D, PY - 1981/6/1/pubmed PY - 1981/6/1/medline PY - 1981/6/1/entrez SP - 426 EP - 34 JF - The Journal of allergy and clinical immunology JO - J Allergy Clin Immunol VL - 67 IS - 6 N2 - Study of the release of slow-reacting substance of anaphylaxis (SRS-A) from lung cells has been hampered by the lack of suitable animal model. Using both immunologic and pharmacologic stimuli, we have obtained histamine and SRS-A release from dispersed pig lung cells containing 6% mast cells (with a histamine content of 1.9 pg/cell). Lung cells dispersed from actively sensitized (with intratracheal Ascaris antigen) but not unsensitized pigs released both histamine (mean net release 33%) and SRS-A (mean release, 47 units/10(7) cells) when challenged with Ascaris antigen. Greater release of histamine (mean net release 52%) and of slow-reacting substance (SRS) (mean release 701 units/10(7) cells) was induced by challenge with the calcium ionophore A23187. The pharmacologic and physicochemical characteristics of the SRS together with its profile of enzymatic inactivation resembled those described for SRA-A released from human lung. Both antigen-induced and A23187-induced SRS(-A) release were enhanced by indomethacin, a cyclo-oxygenase inhibitor, but inhibited by both phenidone (IC50 35 microM) and eicosatetraenoic acid (IC50 15 microM), inhibitors of both cyclo-oxygenase and lipoxygenase, confirming that generation of SRS(-A) by either stimulus required an intact lipoxygenase pathway of arachidonic acid metabolism. SN - 0091-6749 UR - https://www.unboundmedicine.com/medline/citation/6809801/Release_of_slow_reacting_substance_of_anaphylaxis_from_dispersed_pig_lung_cells:_effect_of_cyclo_oxygenase_and_lipoxygenase_inhibitors_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0091-6749(81)90095-6 DB - PRIME DP - Unbound Medicine ER -