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Quantitation of Fc receptors and surface immunoglobulin is affected by cell isolation procedures using plasmagel and ficoll-hypaque.
J Immunol Methods. 1978; 22(3-4):263-72.JI

Abstract

When mononuclear leukocytes are isolated directly from whole human blood using Ficoll-Hypaque or Plasmagel, cytophilic immunoglobulin is detected on cell surfaces. Upon incubation at 37 degrees C, this cell-associated immunoglobulin is shed slowly into the medium. However, when cells are prewashed in phosphate-buffered saline prior to isolation, they appear to be free of cytophilic immunoglobulin. Compared to prewashed cells, populations retaining cytophilic immunoglobulin on their surfaces demonstrate a decreased binding of soluble immune complexes and radiolabelled trimeric rabbit IgG. The data suggest that Ficoll-Hypaque and Plasmagel cause serum IgG to bind with abnormally high affinity to human mononuclear leukocytes, probably via Fc receptors. This artifact of preparation can lead to erroneous estimates of the numbers of cells bearing Fc receptors or intrinsic membrane immunoglobulin within a given population of cells and to an inaccurate assessment of the average number of Fc receptors per cell.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

681759

Citation

Alexander, E L., et al. "Quantitation of Fc Receptors and Surface Immunoglobulin Is Affected By Cell Isolation Procedures Using Plasmagel and Ficoll-hypaque." Journal of Immunological Methods, vol. 22, no. 3-4, 1978, pp. 263-72.
Alexander EL, Titus JA, Segal DM. Quantitation of Fc receptors and surface immunoglobulin is affected by cell isolation procedures using plasmagel and ficoll-hypaque. J Immunol Methods. 1978;22(3-4):263-72.
Alexander, E. L., Titus, J. A., & Segal, D. M. (1978). Quantitation of Fc receptors and surface immunoglobulin is affected by cell isolation procedures using plasmagel and ficoll-hypaque. Journal of Immunological Methods, 22(3-4), 263-72.
Alexander EL, Titus JA, Segal DM. Quantitation of Fc Receptors and Surface Immunoglobulin Is Affected By Cell Isolation Procedures Using Plasmagel and Ficoll-hypaque. J Immunol Methods. 1978;22(3-4):263-72. PubMed PMID: 681759.
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TY - JOUR T1 - Quantitation of Fc receptors and surface immunoglobulin is affected by cell isolation procedures using plasmagel and ficoll-hypaque. AU - Alexander,E L, AU - Titus,J A, AU - Segal,D M, PY - 1978/1/1/pubmed PY - 1978/1/1/medline PY - 1978/1/1/entrez SP - 263 EP - 72 JF - Journal of immunological methods JO - J. Immunol. Methods VL - 22 IS - 3-4 N2 - When mononuclear leukocytes are isolated directly from whole human blood using Ficoll-Hypaque or Plasmagel, cytophilic immunoglobulin is detected on cell surfaces. Upon incubation at 37 degrees C, this cell-associated immunoglobulin is shed slowly into the medium. However, when cells are prewashed in phosphate-buffered saline prior to isolation, they appear to be free of cytophilic immunoglobulin. Compared to prewashed cells, populations retaining cytophilic immunoglobulin on their surfaces demonstrate a decreased binding of soluble immune complexes and radiolabelled trimeric rabbit IgG. The data suggest that Ficoll-Hypaque and Plasmagel cause serum IgG to bind with abnormally high affinity to human mononuclear leukocytes, probably via Fc receptors. This artifact of preparation can lead to erroneous estimates of the numbers of cells bearing Fc receptors or intrinsic membrane immunoglobulin within a given population of cells and to an inaccurate assessment of the average number of Fc receptors per cell. SN - 0022-1759 UR - https://www.unboundmedicine.com/medline/citation/681759/Quantitation_of_Fc_receptors_and_surface_immunoglobulin_is_affected_by_cell_isolation_procedures_using_plasmagel_and_ficoll-hypaque L2 - https://linkinghub.elsevier.com/retrieve/pii/0022-1759(78)90034-0 DB - PRIME DP - Unbound Medicine ER -