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Diagnosis of histoplasmosis and blastomycosis by an antiglobulin hemagglutination test.
Am J Vet Res. 1982 Dec; 43(12):2279-83.AJ

Abstract

An antiglobulin hemagglutination test was developed for detection of antibody directed to Histoplasma capsulatum and Blastomyces dermatitidis. The substances responsible for spontaneous agglutination of erythrocytes were removed from histoplasmin and blastomycin by vacuum dialysis, and partial purification of the antigens by gel filtration chromatography on Sephadex G-150 allowed removal of additional nonantigenic material which competed with the antigens for binding on the erythrocyte surface. The test was sensitive enough to detect antibodies in sera which were negative by complement fixation, immunodiffusion, or both, but it failed to discriminate reliably between antibody directed to H capsulatum and antibody directed to B dermatitidis. Erythrocytes sensitized with partially purified blastomycin produced some false-positive reactions with normal canine sera; this was corrected by diluting the antigen before sensitization of the erythrocytes.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

6819794

Citation

Khansari, N, et al. "Diagnosis of Histoplasmosis and Blastomycosis By an Antiglobulin Hemagglutination Test." American Journal of Veterinary Research, vol. 43, no. 12, 1982, pp. 2279-83.
Khansari N, Segre D, Segre M. Diagnosis of histoplasmosis and blastomycosis by an antiglobulin hemagglutination test. Am J Vet Res. 1982;43(12):2279-83.
Khansari, N., Segre, D., & Segre, M. (1982). Diagnosis of histoplasmosis and blastomycosis by an antiglobulin hemagglutination test. American Journal of Veterinary Research, 43(12), 2279-83.
Khansari N, Segre D, Segre M. Diagnosis of Histoplasmosis and Blastomycosis By an Antiglobulin Hemagglutination Test. Am J Vet Res. 1982;43(12):2279-83. PubMed PMID: 6819794.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diagnosis of histoplasmosis and blastomycosis by an antiglobulin hemagglutination test. AU - Khansari,N, AU - Segre,D, AU - Segre,M, PY - 1982/12/1/pubmed PY - 1982/12/1/medline PY - 1982/12/1/entrez SP - 2279 EP - 83 JF - American journal of veterinary research JO - Am J Vet Res VL - 43 IS - 12 N2 - An antiglobulin hemagglutination test was developed for detection of antibody directed to Histoplasma capsulatum and Blastomyces dermatitidis. The substances responsible for spontaneous agglutination of erythrocytes were removed from histoplasmin and blastomycin by vacuum dialysis, and partial purification of the antigens by gel filtration chromatography on Sephadex G-150 allowed removal of additional nonantigenic material which competed with the antigens for binding on the erythrocyte surface. The test was sensitive enough to detect antibodies in sera which were negative by complement fixation, immunodiffusion, or both, but it failed to discriminate reliably between antibody directed to H capsulatum and antibody directed to B dermatitidis. Erythrocytes sensitized with partially purified blastomycin produced some false-positive reactions with normal canine sera; this was corrected by diluting the antigen before sensitization of the erythrocytes. SN - 0002-9645 UR - https://www.unboundmedicine.com/medline/citation/6819794/Diagnosis_of_histoplasmosis_and_blastomycosis_by_an_antiglobulin_hemagglutination_test_ L2 - http://www.diseaseinfosearch.org/result/864 DB - PRIME DP - Unbound Medicine ER -