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Studies on synthetic chromatins containing poly(dA-dT) X poly(dA-dT) and poly(dG-dC) X poly(dG-dC).
Biochim Biophys Acta. 1983 Jan 20; 739(1):85-96.BB

Abstract

Core histones (H2A,H2B,H3,H4)2, were reconstituted with the synthetic polynucleotides poly(dA-dT) X poly(dA-dT) and poly(dG-dC) X poly(dG-dC) to yield synthetic chromatins containing 200 basepairs per octamer. These synthetic chromatins displayed a 36% decrease in the circular dichroism (CD) peak ellipticity from the value of the polynucleotide free in solution; the poly(dA-dT) X poly(dA-dT)/chromatin showed an increase in the complexity of the thermal denaturation profile compared to that of the polynucleotide. Both the temperature of maximum dh/dT for each transition (Tm) and the relative amount of poly(dA-dT) X poly(dA-dT) in the synthetic chromatin melting in each of the four thermal transitions is a function of the ionic strength over the 0-5 mM sodium phosphate range (0.25 mM EDTA, pH 7.0); a shift of material toward higher melting transitions was observed with increasing ionic strength. The CD peak ellipticity value for both synthetic chromatins was ionic strength-independent over the 0-5 mM sodium phosphate range. These results are in contrast to those observed with H1/H5 stripped chicken erythrocyte chromatin (Fulmer, A. and Fasman, G.D. (1979) Biopolymers 18, 2875-2891), where an ionic strength dependence was found. Differences in the CD spectra between poly(dA-dT) X poly(dA-dT)/chromatin, poly(dG-dC) X poly(dG-dC)/chromatin and H1/H5 stripped chicken erythrocyte chromatin suggest subtle differences in assembly. Finally, the temperature dependence of the CD spectra of poly(dA-dT) X poly(dA-dT)-containing synthetic chromatin, which is similar to that for the polynucleotide, suggests the core histone bound polynucleotide has a large degree of conformational flexibility allowing it to undergo the premelt transition.

Authors

No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

6830804

Citation

Prevelige, P E., and G D. Fasman. "Studies On Synthetic Chromatins Containing poly(dA-dT) X poly(dA-dT) and poly(dG-dC) X Poly(dG-dC)." Biochimica Et Biophysica Acta, vol. 739, no. 1, 1983, pp. 85-96.
Prevelige PE, Fasman GD. Studies on synthetic chromatins containing poly(dA-dT) X poly(dA-dT) and poly(dG-dC) X poly(dG-dC). Biochim Biophys Acta. 1983;739(1):85-96.
Prevelige, P. E., & Fasman, G. D. (1983). Studies on synthetic chromatins containing poly(dA-dT) X poly(dA-dT) and poly(dG-dC) X poly(dG-dC). Biochimica Et Biophysica Acta, 739(1), 85-96.
Prevelige PE, Fasman GD. Studies On Synthetic Chromatins Containing poly(dA-dT) X poly(dA-dT) and poly(dG-dC) X Poly(dG-dC). Biochim Biophys Acta. 1983 Jan 20;739(1):85-96. PubMed PMID: 6830804.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Studies on synthetic chromatins containing poly(dA-dT) X poly(dA-dT) and poly(dG-dC) X poly(dG-dC). AU - Prevelige,P E,Jr AU - Fasman,G D, PY - 1983/1/20/pubmed PY - 2001/3/28/medline PY - 1983/1/20/entrez SP - 85 EP - 96 JF - Biochimica et biophysica acta JO - Biochim. Biophys. Acta VL - 739 IS - 1 N2 - Core histones (H2A,H2B,H3,H4)2, were reconstituted with the synthetic polynucleotides poly(dA-dT) X poly(dA-dT) and poly(dG-dC) X poly(dG-dC) to yield synthetic chromatins containing 200 basepairs per octamer. These synthetic chromatins displayed a 36% decrease in the circular dichroism (CD) peak ellipticity from the value of the polynucleotide free in solution; the poly(dA-dT) X poly(dA-dT)/chromatin showed an increase in the complexity of the thermal denaturation profile compared to that of the polynucleotide. Both the temperature of maximum dh/dT for each transition (Tm) and the relative amount of poly(dA-dT) X poly(dA-dT) in the synthetic chromatin melting in each of the four thermal transitions is a function of the ionic strength over the 0-5 mM sodium phosphate range (0.25 mM EDTA, pH 7.0); a shift of material toward higher melting transitions was observed with increasing ionic strength. The CD peak ellipticity value for both synthetic chromatins was ionic strength-independent over the 0-5 mM sodium phosphate range. These results are in contrast to those observed with H1/H5 stripped chicken erythrocyte chromatin (Fulmer, A. and Fasman, G.D. (1979) Biopolymers 18, 2875-2891), where an ionic strength dependence was found. Differences in the CD spectra between poly(dA-dT) X poly(dA-dT)/chromatin, poly(dG-dC) X poly(dG-dC)/chromatin and H1/H5 stripped chicken erythrocyte chromatin suggest subtle differences in assembly. Finally, the temperature dependence of the CD spectra of poly(dA-dT) X poly(dA-dT)-containing synthetic chromatin, which is similar to that for the polynucleotide, suggests the core histone bound polynucleotide has a large degree of conformational flexibility allowing it to undergo the premelt transition. SN - 0006-3002 UR - https://www.unboundmedicine.com/medline/citation/6830804/Studies_on_synthetic_chromatins_containing_poly_dA_dT__X_poly_dA_dT__and_poly_dG_dC__X_poly_dG_dC__ L2 - https://linkinghub.elsevier.com/retrieve/pii/0167-4781(83)90048-9 DB - PRIME DP - Unbound Medicine ER -