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A radioimmunoassay for alpha- and beta-gliadins.
Clin Sci (Lond). 1983 Jun; 64(6):655-9.CS

Abstract

1. A rapid, sensitive specific radioimmunoassay for alpha- and beta-gliadin has been developed using an antiserum raised in rabbits to A-gliadin, a component of alpha-gliadin. 2. The antigen used in the assay was alpha-gliadin labelled with 125I; antigen-antibody complexes were collected after adsorption to Staphylococcus aureus in suspension. 3. The sensitivity of the assay, as judged by competitive binding with unlabelled antigen, was 1 ng of alpha- or beta-gliadin, which show complete cross-reaction with this antiserum. 4. Cross-reactivity to other wheat proteins was less than 1% and no cross-reactivity to extracts of rye, barley or oats was observed. 5. This radioimmunoassay for alpha- and beta-gliadin has been used to measure their amount in different varieties of wheat flour, several foods prepared from flour, e.g. bread, biscuits and products prepared as 'gluten free'. The possibility of assaying for alpha-gliadin in prepared foods is of special value since alpha-, beta-, gamma- and omega-gliadin have been shown to exacerbate coeliac disease.

Authors

No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

6839670

Citation

Ciclitira, P J., and E S. Lennox. "A Radioimmunoassay for Alpha- and Beta-gliadins." Clinical Science (London, England : 1979), vol. 64, no. 6, 1983, pp. 655-9.
Ciclitira PJ, Lennox ES. A radioimmunoassay for alpha- and beta-gliadins. Clin Sci. 1983;64(6):655-9.
Ciclitira, P. J., & Lennox, E. S. (1983). A radioimmunoassay for alpha- and beta-gliadins. Clinical Science (London, England : 1979), 64(6), 655-9.
Ciclitira PJ, Lennox ES. A Radioimmunoassay for Alpha- and Beta-gliadins. Clin Sci. 1983;64(6):655-9. PubMed PMID: 6839670.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A radioimmunoassay for alpha- and beta-gliadins. AU - Ciclitira,P J, AU - Lennox,E S, PY - 1983/6/1/pubmed PY - 1983/6/1/medline PY - 1983/6/1/entrez SP - 655 EP - 9 JF - Clinical science (London, England : 1979) JO - Clin. Sci. VL - 64 IS - 6 N2 - 1. A rapid, sensitive specific radioimmunoassay for alpha- and beta-gliadin has been developed using an antiserum raised in rabbits to A-gliadin, a component of alpha-gliadin. 2. The antigen used in the assay was alpha-gliadin labelled with 125I; antigen-antibody complexes were collected after adsorption to Staphylococcus aureus in suspension. 3. The sensitivity of the assay, as judged by competitive binding with unlabelled antigen, was 1 ng of alpha- or beta-gliadin, which show complete cross-reaction with this antiserum. 4. Cross-reactivity to other wheat proteins was less than 1% and no cross-reactivity to extracts of rye, barley or oats was observed. 5. This radioimmunoassay for alpha- and beta-gliadin has been used to measure their amount in different varieties of wheat flour, several foods prepared from flour, e.g. bread, biscuits and products prepared as 'gluten free'. The possibility of assaying for alpha-gliadin in prepared foods is of special value since alpha-, beta-, gamma- and omega-gliadin have been shown to exacerbate coeliac disease. SN - 0143-5221 UR - https://www.unboundmedicine.com/medline/citation/6839670/A_radioimmunoassay_for_alpha__and_beta_gliadins_ L2 - https://portlandpress.com/clinsci/article-lookup/doi/10.1042/cs0640655 DB - PRIME DP - Unbound Medicine ER -