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Changes in synaptic membrane order associated with chronic ethanol treatment in mice.
Mol Pharmacol. 1983 Jan; 23(1):86-91.MP

Abstract

The fluidity of brain synaptosomal plasma membranes (SPM) from ethanol-treated mice and control mice was investigated by a sensitive EPR technique. Mice were made tolerant to and physically dependent on ethanol by 3 days of continuous exposure to ethanol vapor. Daily injections of pyrazole were used to stabilize the blood alcohol levels. At the time of withdrawal, SPM were prepared and spin-labeled with 5-, 12-, or 16-doxylstearic acid. "Baseline" order parameters were measured in the absence of added alcohol. The chronic ethanol treatment produced a significant increase in the baseline order parameter that could be detected with the 12-doxyl probe. This indicates a more rigid membrane associated with the withdrawal hyperexicitability. Baseline order parameters measured with the 5-doxyl probe (near the bilayer surface) or with the 16-doxyl probe (near the bilayer interior) were not affected by the chronic ethanol treatment. When ethanol or t-butanol was added in vitro to membranes labeled with the 5- or 12-doxyl probe, a membrane component of tolerance was revealed; that is, the SPM from ethanol-treated mice required more alcohol to reduce the membrane to a given disordered state. The SPM content of cholesterol and phospholipid was not affected by the chronic ethanol treatment.

Authors

No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

6865903

Citation

Lyon, R C., and D B. Goldstein. "Changes in Synaptic Membrane Order Associated With Chronic Ethanol Treatment in Mice." Molecular Pharmacology, vol. 23, no. 1, 1983, pp. 86-91.
Lyon RC, Goldstein DB. Changes in synaptic membrane order associated with chronic ethanol treatment in mice. Mol Pharmacol. 1983;23(1):86-91.
Lyon, R. C., & Goldstein, D. B. (1983). Changes in synaptic membrane order associated with chronic ethanol treatment in mice. Molecular Pharmacology, 23(1), 86-91.
Lyon RC, Goldstein DB. Changes in Synaptic Membrane Order Associated With Chronic Ethanol Treatment in Mice. Mol Pharmacol. 1983;23(1):86-91. PubMed PMID: 6865903.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Changes in synaptic membrane order associated with chronic ethanol treatment in mice. AU - Lyon,R C, AU - Goldstein,D B, PY - 1983/1/1/pubmed PY - 1983/1/1/medline PY - 1983/1/1/entrez SP - 86 EP - 91 JF - Molecular pharmacology JO - Mol Pharmacol VL - 23 IS - 1 N2 - The fluidity of brain synaptosomal plasma membranes (SPM) from ethanol-treated mice and control mice was investigated by a sensitive EPR technique. Mice were made tolerant to and physically dependent on ethanol by 3 days of continuous exposure to ethanol vapor. Daily injections of pyrazole were used to stabilize the blood alcohol levels. At the time of withdrawal, SPM were prepared and spin-labeled with 5-, 12-, or 16-doxylstearic acid. "Baseline" order parameters were measured in the absence of added alcohol. The chronic ethanol treatment produced a significant increase in the baseline order parameter that could be detected with the 12-doxyl probe. This indicates a more rigid membrane associated with the withdrawal hyperexicitability. Baseline order parameters measured with the 5-doxyl probe (near the bilayer surface) or with the 16-doxyl probe (near the bilayer interior) were not affected by the chronic ethanol treatment. When ethanol or t-butanol was added in vitro to membranes labeled with the 5- or 12-doxyl probe, a membrane component of tolerance was revealed; that is, the SPM from ethanol-treated mice required more alcohol to reduce the membrane to a given disordered state. The SPM content of cholesterol and phospholipid was not affected by the chronic ethanol treatment. SN - 0026-895X UR - https://www.unboundmedicine.com/medline/citation/6865903/Changes_in_synaptic_membrane_order_associated_with_chronic_ethanol_treatment_in_mice_ L2 - http://molpharm.aspetjournals.org/cgi/pmidlookup?view=long&pmid=6865903 DB - PRIME DP - Unbound Medicine ER -