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Construction of a mobilizable Yersinia enterocolitica virulence plasmid.
J Bacteriol 1983; 155(2):761-7JB

Abstract

Virulence of Yersinia enterocolitica O:8 is associated with pO:8, a 42-megadalton plasmid. We constructed a mobilizable pO:8 derivative by successive in vitro and in vivo genetic manipulations. The in vitro constructed hybrid molecule pRK290B8-5 consisting of the mobilizable vector pRK290B and a 2.9-megadalton BamHI fragment of pO:8 was conjugally transferred to a Y. enterocolitica strain of serotype O:8 which harbored the virulence plasmid pO:8. From Yersinia transconjugants, a cointegrate was isolated which apparently formed by homologous recombination between the two component plasmids. The cointegrate was mobilized into plasmidless Y. enterocolitica strains of different serotypes. The transconjugants of serotype O:8 were found to express all four plasmid-associated phenotypes: (i) mouse lethality (Ml), (ii) conjunctivitis provocation in the guinea pig eye (Con), (iii) calcium requirement for growth at 37 degrees C (Mox), and (iv) agglutinogens (Ag8). The transconjugants of serotype O:3 expressed the phenotypes Con, Mox, and Ag8 but were nonlethal for mice (Ml-). The transconjugants of serotype O:5 remained avirulent for mice (Ml-) and for the guinea pig eye (Con-) but expressed the phenotypes Mox and Ag8. These data show that the virulence plasmid is probably not functionally interchangeable within different serotypes of Y. enterocolitica.

Authors

No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

6874644

Citation

Heesemann, J, and R Laufs. "Construction of a Mobilizable Yersinia Enterocolitica Virulence Plasmid." Journal of Bacteriology, vol. 155, no. 2, 1983, pp. 761-7.
Heesemann J, Laufs R. Construction of a mobilizable Yersinia enterocolitica virulence plasmid. J Bacteriol. 1983;155(2):761-7.
Heesemann, J., & Laufs, R. (1983). Construction of a mobilizable Yersinia enterocolitica virulence plasmid. Journal of Bacteriology, 155(2), pp. 761-7.
Heesemann J, Laufs R. Construction of a Mobilizable Yersinia Enterocolitica Virulence Plasmid. J Bacteriol. 1983;155(2):761-7. PubMed PMID: 6874644.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Construction of a mobilizable Yersinia enterocolitica virulence plasmid. AU - Heesemann,J, AU - Laufs,R, PY - 1983/8/1/pubmed PY - 1983/8/1/medline PY - 1983/8/1/entrez SP - 761 EP - 7 JF - Journal of bacteriology JO - J. Bacteriol. VL - 155 IS - 2 N2 - Virulence of Yersinia enterocolitica O:8 is associated with pO:8, a 42-megadalton plasmid. We constructed a mobilizable pO:8 derivative by successive in vitro and in vivo genetic manipulations. The in vitro constructed hybrid molecule pRK290B8-5 consisting of the mobilizable vector pRK290B and a 2.9-megadalton BamHI fragment of pO:8 was conjugally transferred to a Y. enterocolitica strain of serotype O:8 which harbored the virulence plasmid pO:8. From Yersinia transconjugants, a cointegrate was isolated which apparently formed by homologous recombination between the two component plasmids. The cointegrate was mobilized into plasmidless Y. enterocolitica strains of different serotypes. The transconjugants of serotype O:8 were found to express all four plasmid-associated phenotypes: (i) mouse lethality (Ml), (ii) conjunctivitis provocation in the guinea pig eye (Con), (iii) calcium requirement for growth at 37 degrees C (Mox), and (iv) agglutinogens (Ag8). The transconjugants of serotype O:3 expressed the phenotypes Con, Mox, and Ag8 but were nonlethal for mice (Ml-). The transconjugants of serotype O:5 remained avirulent for mice (Ml-) and for the guinea pig eye (Con-) but expressed the phenotypes Mox and Ag8. These data show that the virulence plasmid is probably not functionally interchangeable within different serotypes of Y. enterocolitica. SN - 0021-9193 UR - https://www.unboundmedicine.com/medline/citation/6874644/Construction_of_a_mobilizable_Yersinia_enterocolitica_virulence_plasmid_ L2 - http://jb.asm.org/cgi/pmidlookup?view=long&pmid=6874644 DB - PRIME DP - Unbound Medicine ER -