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Studies on antitumor polysaccharides of Flammulina velutipes (Curt. ex Fr.) Sing.II. The structure of EA3 and further purification of EA5.
J Pharmacobiodyn. 1982 Aug; 5(8):576-81.JP

Abstract

An antitumor polysaccharide, EA3 isolated from a Japanese edible mushroom, Flammulina velutipes (CURT. ex FR.) SING. is composed of D-glucose. Studies to determine its structure were performed by mean of partial acid hydrolysis, acetolysis and the chemical analysis of the complete hydrolysates of the fully methylated polysaccharide. Thus the chemical structure of EA3 was found to be that of a beta-(1 leads to 3)-glucan. Another antitumor polysaccharide (EA5) also isolated from F. velutipes was fractionated on a concanavalin A-Sepharose 4B column. The active antitumor component was further purified by column chromatography on Sephadex G-200. Among the polysaccharides isolated the highest molecular weight polysaccharide (EA501) showed the highest rate of antitumor activity. The component sugar of EA501 were found to be D-glucose 42.3%, D-galactose 17.3%, D-mannose 12.2%, D-xylose 6.7% and L-arabinose 14.7%.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

6891400

Citation

Ikekawa, T, et al. "Studies On Antitumor Polysaccharides of Flammulina Velutipes (Curt. Ex Fr.) Sing.II. the Structure of EA3 and Further Purification of EA5." Journal of Pharmacobio-dynamics, vol. 5, no. 8, 1982, pp. 576-81.
Ikekawa T, Ikeda Y, Yoshioka Y, et al. Studies on antitumor polysaccharides of Flammulina velutipes (Curt. ex Fr.) Sing.II. The structure of EA3 and further purification of EA5. J Pharmacobiodyn. 1982;5(8):576-81.
Ikekawa, T., Ikeda, Y., Yoshioka, Y., Nakanishi, K., Yokoyama, E., & Yamazaki, E. (1982). Studies on antitumor polysaccharides of Flammulina velutipes (Curt. ex Fr.) Sing.II. The structure of EA3 and further purification of EA5. Journal of Pharmacobio-dynamics, 5(8), 576-81.
Ikekawa T, et al. Studies On Antitumor Polysaccharides of Flammulina Velutipes (Curt. Ex Fr.) Sing.II. the Structure of EA3 and Further Purification of EA5. J Pharmacobiodyn. 1982;5(8):576-81. PubMed PMID: 6891400.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Studies on antitumor polysaccharides of Flammulina velutipes (Curt. ex Fr.) Sing.II. The structure of EA3 and further purification of EA5. AU - Ikekawa,T, AU - Ikeda,Y, AU - Yoshioka,Y, AU - Nakanishi,K, AU - Yokoyama,E, AU - Yamazaki,E, PY - 1982/8/1/pubmed PY - 1982/8/1/medline PY - 1982/8/1/entrez SP - 576 EP - 81 JF - Journal of pharmacobio-dynamics JO - J Pharmacobiodyn VL - 5 IS - 8 N2 - An antitumor polysaccharide, EA3 isolated from a Japanese edible mushroom, Flammulina velutipes (CURT. ex FR.) SING. is composed of D-glucose. Studies to determine its structure were performed by mean of partial acid hydrolysis, acetolysis and the chemical analysis of the complete hydrolysates of the fully methylated polysaccharide. Thus the chemical structure of EA3 was found to be that of a beta-(1 leads to 3)-glucan. Another antitumor polysaccharide (EA5) also isolated from F. velutipes was fractionated on a concanavalin A-Sepharose 4B column. The active antitumor component was further purified by column chromatography on Sephadex G-200. Among the polysaccharides isolated the highest molecular weight polysaccharide (EA501) showed the highest rate of antitumor activity. The component sugar of EA501 were found to be D-glucose 42.3%, D-galactose 17.3%, D-mannose 12.2%, D-xylose 6.7% and L-arabinose 14.7%. SN - 0386-846X UR - https://www.unboundmedicine.com/medline/citation/6891400/Studies_on_antitumor_polysaccharides_of_Flammulina_velutipes__Curt__ex_Fr___Sing_II__The_structure_of_EA3_and_further_purification_of_EA5_ DB - PRIME DP - Unbound Medicine ER -