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Concanavalin A induced histamine release from human basophils in vitro.
Immunology. 1977 Jul; 33(1):51-8.I

Abstract

The site of interaction for concanavalin A (Con-A)-induced histamine release from human basophils was studied in vitro. Blocking the epsilon one determinant (D leads to 1) of IgE with high concentrations of monomer (Fab) anti-Depsilon1 does not significantly inhibit the quantity of histamine released by suboptimum concentrations of Fc specific anti-IgE. This indicates that the monomer anti-Depsilon1 does not have the capacity to sterically hinder the bridging of all of the determinants in the Cepsilon3 and Cepsilon4 domains (Fc'-epsilon-region) of IgE. The monomer anti-Depsilon1 does effectively inhibit release induced by suboptimum concentrations of Con-A. The data indicate that for suboptimum concentrations, Con-A activation is IgE mediated and takes place in the proximity of Depsilon1 and not at the membrane receptor for IgE.

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Authors

Magro AM
No affiliation info available
Bennich H
No affiliation info available

MeSH

BasophilsBinding Sites, AntibodyComplement C3Complement C4Concanavalin ADose-Response Relationship, ImmunologicEpitopesHistamine ReleaseHumansImmunoglobulin EImmunoglobulin Fc FragmentsIn Vitro Techniques

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

68925

Citation

Magro, A M., and H Bennich. "Concanavalin a Induced Histamine Release From Human Basophils in Vitro." Immunology, vol. 33, no. 1, 1977, pp. 51-8.
Magro AM, Bennich H. Concanavalin A induced histamine release from human basophils in vitro. Immunology. 1977;33(1):51-8.
Magro, A. M., & Bennich, H. (1977). Concanavalin A induced histamine release from human basophils in vitro. Immunology, 33(1), 51-8.
Magro AM, Bennich H. Concanavalin a Induced Histamine Release From Human Basophils in Vitro. Immunology. 1977;33(1):51-8. PubMed PMID: 68925.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Concanavalin A induced histamine release from human basophils in vitro. AU - Magro,A M, AU - Bennich,H, PY - 1977/7/1/pubmed PY - 1977/7/1/medline PY - 1977/7/1/entrez SP - 51 EP - 8 JF - Immunology JO - Immunology VL - 33 IS - 1 N2 - The site of interaction for concanavalin A (Con-A)-induced histamine release from human basophils was studied in vitro. Blocking the epsilon one determinant (D leads to 1) of IgE with high concentrations of monomer (Fab) anti-Depsilon1 does not significantly inhibit the quantity of histamine released by suboptimum concentrations of Fc specific anti-IgE. This indicates that the monomer anti-Depsilon1 does not have the capacity to sterically hinder the bridging of all of the determinants in the Cepsilon3 and Cepsilon4 domains (Fc'-epsilon-region) of IgE. The monomer anti-Depsilon1 does effectively inhibit release induced by suboptimum concentrations of Con-A. The data indicate that for suboptimum concentrations, Con-A activation is IgE mediated and takes place in the proximity of Depsilon1 and not at the membrane receptor for IgE. SN - 0019-2805 UR - https://www.unboundmedicine.com/medline/citation/68925/Concanavalin_A_induced_histamine_release_from_human_basophils_in_vitro_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/68925/ DB - PRIME DP - Unbound Medicine ER -