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Genetic analysis of bacteriophage P4 using P4-plasmid ColE1 hybrids.
Mol Gen Genet. 1980 Feb; 177(3):399-412.MG

Abstract

A set of plasmids that contain fragments of the bacteriophage P4 genome has been constructed by deleting portions of a P4-ColE1 hybrid. A P4 genetic map has been established and related to the physical map by examining the ability of these plasmids to rescue various P4 mutations. The P4 virl mutation and P4 genes involved in DNA replication (alpha), activation of P2 helper genes (delta and epsilon), polarity suppression (psu) and head size determination (sid) have been mapped, as has the region responsible for synthesis of a nonessential P4 protein. One of the deleted plasmids contains only 5900 base pairs (52%) of P4 but will form plaques if additional DNA is added to increase its total size to near that of P4. This plasmid is also unique in that it will not form stable associations with P2 lysogens of E. coli which are recA+. P4 alpha mutants can be suppressed as a result of replication under control of the ColE1 part of the hybrid.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

6929401

Citation

Kahn, M, et al. "Genetic Analysis of Bacteriophage P4 Using P4-plasmid ColE1 Hybrids." Molecular & General Genetics : MGG, vol. 177, no. 3, 1980, pp. 399-412.
Kahn M, Ow D, Sauer B, et al. Genetic analysis of bacteriophage P4 using P4-plasmid ColE1 hybrids. Mol Gen Genet. 1980;177(3):399-412.
Kahn, M., Ow, D., Sauer, B., Rabinowitz, A., & Calendar, R. (1980). Genetic analysis of bacteriophage P4 using P4-plasmid ColE1 hybrids. Molecular & General Genetics : MGG, 177(3), 399-412.
Kahn M, et al. Genetic Analysis of Bacteriophage P4 Using P4-plasmid ColE1 Hybrids. Mol Gen Genet. 1980;177(3):399-412. PubMed PMID: 6929401.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genetic analysis of bacteriophage P4 using P4-plasmid ColE1 hybrids. AU - Kahn,M, AU - Ow,D, AU - Sauer,B, AU - Rabinowitz,A, AU - Calendar,R, PY - 1980/2/1/pubmed PY - 1980/2/1/medline PY - 1980/2/1/entrez SP - 399 EP - 412 JF - Molecular & general genetics : MGG JO - Mol. Gen. Genet. VL - 177 IS - 3 N2 - A set of plasmids that contain fragments of the bacteriophage P4 genome has been constructed by deleting portions of a P4-ColE1 hybrid. A P4 genetic map has been established and related to the physical map by examining the ability of these plasmids to rescue various P4 mutations. The P4 virl mutation and P4 genes involved in DNA replication (alpha), activation of P2 helper genes (delta and epsilon), polarity suppression (psu) and head size determination (sid) have been mapped, as has the region responsible for synthesis of a nonessential P4 protein. One of the deleted plasmids contains only 5900 base pairs (52%) of P4 but will form plaques if additional DNA is added to increase its total size to near that of P4. This plasmid is also unique in that it will not form stable associations with P2 lysogens of E. coli which are recA+. P4 alpha mutants can be suppressed as a result of replication under control of the ColE1 part of the hybrid. SN - 0026-8925 UR - https://www.unboundmedicine.com/medline/citation/6929401/Genetic_analysis_of_bacteriophage_P4_using_P4_plasmid_ColE1_hybrids_ L2 - https://doi.org/10.1007/BF00271478 DB - PRIME DP - Unbound Medicine ER -