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Experimental allergic encephalomyelitis supernatant transfer activity (EAE-STA) in Lewis rats:immunobiologic and initial biochemical properties.
J Immunol. 1980 Apr; 124(4):1784-8.JI

Abstract

Supernatants derived from incubated lymph node cells (LNC) of Lewis rats sensitized to neuroantigen-adjuvant have the capacity to transfer experimental allergic encephalomyelitis (EAE) to syngeneic recipients. Sensitization of donors with guinea pig or rat spinal cord adjuvant by either of two routes of injection was effective in generating EAE supernatant transfer activity (EAE-STA). Despite transfer of typical EAE histopathologic lesions, which were intense and disseminated throughout the neuraxis in some animals, recipient animals invariably remained clinically well. Donor sensitization with purified myelin basic protein of guinea pig or rat origin was conspicuously ineffective in generating EAE-STA. Absorption studies revealed that EAE-STA was diminished after exposure to guinea pig or rat spinal cord or guinea pig or rat kidney, but it was not demonstrably reduced after absorption with neonatal rat spinal cord lacking encephalitogenic activity or with guinea pig or rat myelin basic protein or lysozyme. EAE-STA is stable at -20 degrees C/4 days, -70 degrees C/2 days, 4 degrees C/18 hr, and 56 degrees C/1 hr, and has a m.w. in excess of 100,000 daltons.

Authors

No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

6965962

Citation

Whitacre, C C., and P Y. Paterson. "Experimental Allergic Encephalomyelitis Supernatant Transfer Activity (EAE-STA) in Lewis Rats:immunobiologic and Initial Biochemical Properties." Journal of Immunology (Baltimore, Md. : 1950), vol. 124, no. 4, 1980, pp. 1784-8.
Whitacre CC, Paterson PY. Experimental allergic encephalomyelitis supernatant transfer activity (EAE-STA) in Lewis rats:immunobiologic and initial biochemical properties. J Immunol. 1980;124(4):1784-8.
Whitacre, C. C., & Paterson, P. Y. (1980). Experimental allergic encephalomyelitis supernatant transfer activity (EAE-STA) in Lewis rats:immunobiologic and initial biochemical properties. Journal of Immunology (Baltimore, Md. : 1950), 124(4), 1784-8.
Whitacre CC, Paterson PY. Experimental Allergic Encephalomyelitis Supernatant Transfer Activity (EAE-STA) in Lewis Rats:immunobiologic and Initial Biochemical Properties. J Immunol. 1980;124(4):1784-8. PubMed PMID: 6965962.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Experimental allergic encephalomyelitis supernatant transfer activity (EAE-STA) in Lewis rats:immunobiologic and initial biochemical properties. AU - Whitacre,C C, AU - Paterson,P Y, PY - 1980/4/1/pubmed PY - 1980/4/1/medline PY - 1980/4/1/entrez SP - 1784 EP - 8 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 124 IS - 4 N2 - Supernatants derived from incubated lymph node cells (LNC) of Lewis rats sensitized to neuroantigen-adjuvant have the capacity to transfer experimental allergic encephalomyelitis (EAE) to syngeneic recipients. Sensitization of donors with guinea pig or rat spinal cord adjuvant by either of two routes of injection was effective in generating EAE supernatant transfer activity (EAE-STA). Despite transfer of typical EAE histopathologic lesions, which were intense and disseminated throughout the neuraxis in some animals, recipient animals invariably remained clinically well. Donor sensitization with purified myelin basic protein of guinea pig or rat origin was conspicuously ineffective in generating EAE-STA. Absorption studies revealed that EAE-STA was diminished after exposure to guinea pig or rat spinal cord or guinea pig or rat kidney, but it was not demonstrably reduced after absorption with neonatal rat spinal cord lacking encephalitogenic activity or with guinea pig or rat myelin basic protein or lysozyme. EAE-STA is stable at -20 degrees C/4 days, -70 degrees C/2 days, 4 degrees C/18 hr, and 56 degrees C/1 hr, and has a m.w. in excess of 100,000 daltons. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/6965962/Experimental_allergic_encephalomyelitis_supernatant_transfer_activity__EAE_STA__in_Lewis_rats:immunobiologic_and_initial_biochemical_properties_ L2 - https://www.jimmunol.org/lookup/pmidlookup?view=long&pmid=6965962 DB - PRIME DP - Unbound Medicine ER -