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Androgen receptor in human skin cytosol.
J Clin Endocrinol Metab. 1981 Feb; 52(2):338-44.JC

Abstract

Human skin, an accessible tissue, is an androgen target organ. We have measured the androgen-binding capacity of human skin cytosol using either 5 alpha[3H]dihydrotestosterone ([3H]DHT) or [3H]methyltrienolone ([3H]R-1881) as ligand. Samples were incubated for 20 h at 0 C, and dextran-coated charcoal was used to separate bound from free steroids. The androgen receptor has a high affinity for both ligands (0.23 +/- 0.04 nM for [3H]DHT; 0.32 +/- 0.16 nM for [3H]R-1881). Testosterone, cyproterone acetate, and, to a lesser extent, estradiol also bind this protein. Progesterone displaces R-1881 from its binding sites, whereas its 5 alpha-reduced metabolite somewhat inhibits DHT binding. The highest binding capacity is measured in cytosol of skin from external genitalia (129.14 +/- 58.0 fmol/g skin; n = 34); it is lower in pubic skin (21.8 +/- 13 fmol/g skin; n = 6). There is no variation as a function of age or sex in genital skin; the higher concentrations observed in the cytosol of pubic skin of women compared to that of men are probably related to lower levels of endogenous steroids. Whereas most patients with the complete form of the testicular feminization syndrome do not have detectable concentrations of androgen receptor, one patient with apparent complete clinical androgen insensitivity had a normal androgen-binding capacity. The parity of values in genital skin from men and women, the absence of variation with age, and the presence of a cytosolic androgen receptor in some androgen-insensitive patients suggest that the androgen receptor in human skin cytosol is not regulated by androgens.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

6970202

Citation

Mowszowicz, I, et al. "Androgen Receptor in Human Skin Cytosol." The Journal of Clinical Endocrinology and Metabolism, vol. 52, no. 2, 1981, pp. 338-44.
Mowszowicz I, Riahi M, Wright F, et al. Androgen receptor in human skin cytosol. J Clin Endocrinol Metab. 1981;52(2):338-44.
Mowszowicz, I., Riahi, M., Wright, F., Bouchard, P., Kuttenn, F., & Mauvais-Jarvis, P. (1981). Androgen receptor in human skin cytosol. The Journal of Clinical Endocrinology and Metabolism, 52(2), 338-44.
Mowszowicz I, et al. Androgen Receptor in Human Skin Cytosol. J Clin Endocrinol Metab. 1981;52(2):338-44. PubMed PMID: 6970202.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Androgen receptor in human skin cytosol. AU - Mowszowicz,I, AU - Riahi,M, AU - Wright,F, AU - Bouchard,P, AU - Kuttenn,F, AU - Mauvais-Jarvis,P, PY - 1981/2/1/pubmed PY - 1981/2/1/medline PY - 1981/2/1/entrez SP - 338 EP - 44 JF - The Journal of clinical endocrinology and metabolism JO - J Clin Endocrinol Metab VL - 52 IS - 2 N2 - Human skin, an accessible tissue, is an androgen target organ. We have measured the androgen-binding capacity of human skin cytosol using either 5 alpha[3H]dihydrotestosterone ([3H]DHT) or [3H]methyltrienolone ([3H]R-1881) as ligand. Samples were incubated for 20 h at 0 C, and dextran-coated charcoal was used to separate bound from free steroids. The androgen receptor has a high affinity for both ligands (0.23 +/- 0.04 nM for [3H]DHT; 0.32 +/- 0.16 nM for [3H]R-1881). Testosterone, cyproterone acetate, and, to a lesser extent, estradiol also bind this protein. Progesterone displaces R-1881 from its binding sites, whereas its 5 alpha-reduced metabolite somewhat inhibits DHT binding. The highest binding capacity is measured in cytosol of skin from external genitalia (129.14 +/- 58.0 fmol/g skin; n = 34); it is lower in pubic skin (21.8 +/- 13 fmol/g skin; n = 6). There is no variation as a function of age or sex in genital skin; the higher concentrations observed in the cytosol of pubic skin of women compared to that of men are probably related to lower levels of endogenous steroids. Whereas most patients with the complete form of the testicular feminization syndrome do not have detectable concentrations of androgen receptor, one patient with apparent complete clinical androgen insensitivity had a normal androgen-binding capacity. The parity of values in genital skin from men and women, the absence of variation with age, and the presence of a cytosolic androgen receptor in some androgen-insensitive patients suggest that the androgen receptor in human skin cytosol is not regulated by androgens. SN - 0021-972X UR - https://www.unboundmedicine.com/medline/citation/6970202/Androgen_receptor_in_human_skin_cytosol_ L2 - https://academic.oup.com/jcem/article-lookup/doi/10.1210/jcem-52-2-338 DB - PRIME DP - Unbound Medicine ER -