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Binding of methyltrienolone to glucocorticoid receptors in rat muscle cytosol.
Endocrinology. 1981 Nov; 109(5):1418-23.E

Abstract

Androgen receptor measurements with [3H]-methyltrienolone [17 beta-hydroxy-17 alpha-methyl-estra-4,9,11-trien-3-one (R1881)] or [3H]testosterone in vastus lateralis muscle cytosol of intact male rats yielded similar numbers of binding sites. Gonadectomy significantly increased the androgen receptor values, and both steroids gave similar results. The binding of [3H]-testosterone was not affected by adrenalectomy. In contrast, [3H]R1881 binding increased 2-fold after adrenalectomy. Competition experiments as well as sucrose density gradient analysis indicated that R1881, in addition to its binding of the androgen receptor, was also bound to glucocorticoid receptor. These results show that the synthetic androgen R1881 must be used with caution in tissues suspected to contain glucocorticoid receptors.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

6975208

Citation

Ho-Kim, M A., et al. "Binding of Methyltrienolone to Glucocorticoid Receptors in Rat Muscle Cytosol." Endocrinology, vol. 109, no. 5, 1981, pp. 1418-23.
Ho-Kim MA, Tremblay RR, Dubé JY. Binding of methyltrienolone to glucocorticoid receptors in rat muscle cytosol. Endocrinology. 1981;109(5):1418-23.
Ho-Kim, M. A., Tremblay, R. R., & Dubé, J. Y. (1981). Binding of methyltrienolone to glucocorticoid receptors in rat muscle cytosol. Endocrinology, 109(5), 1418-23.
Ho-Kim MA, Tremblay RR, Dubé JY. Binding of Methyltrienolone to Glucocorticoid Receptors in Rat Muscle Cytosol. Endocrinology. 1981;109(5):1418-23. PubMed PMID: 6975208.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Binding of methyltrienolone to glucocorticoid receptors in rat muscle cytosol. AU - Ho-Kim,M A, AU - Tremblay,R R, AU - Dubé,J Y, PY - 1981/11/1/pubmed PY - 1981/11/1/medline PY - 1981/11/1/entrez SP - 1418 EP - 23 JF - Endocrinology JO - Endocrinology VL - 109 IS - 5 N2 - Androgen receptor measurements with [3H]-methyltrienolone [17 beta-hydroxy-17 alpha-methyl-estra-4,9,11-trien-3-one (R1881)] or [3H]testosterone in vastus lateralis muscle cytosol of intact male rats yielded similar numbers of binding sites. Gonadectomy significantly increased the androgen receptor values, and both steroids gave similar results. The binding of [3H]-testosterone was not affected by adrenalectomy. In contrast, [3H]R1881 binding increased 2-fold after adrenalectomy. Competition experiments as well as sucrose density gradient analysis indicated that R1881, in addition to its binding of the androgen receptor, was also bound to glucocorticoid receptor. These results show that the synthetic androgen R1881 must be used with caution in tissues suspected to contain glucocorticoid receptors. SN - 0013-7227 UR - https://www.unboundmedicine.com/medline/citation/6975208/Binding_of_methyltrienolone_to_glucocorticoid_receptors_in_rat_muscle_cytosol_ L2 - https://academic.oup.com/endo/article-lookup/doi/10.1210/endo-109-5-1418 DB - PRIME DP - Unbound Medicine ER -