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Replication of ColE1 plasmid deoxyribonucleic acid in a thermosensitive dnaA mutant of Escherichia coli.
J Bacteriol. 1980 Mar; 141(3):1024-30.JB

Abstract

The replication of ColE1 deoxyribonucleic acid (DNA) took place at the restrictive temperature in a dnaA mutant, dnaA167(Ts). It proceeded at a constant rate at 42 degrees C for at least 3 h. The replication was insensitive to rifampin, which blocked replication at the permissive temperature or in the presence of chloramphenicol, even at the restrictive temperature. A linear DNA strand of ColE1 longer than unit genome size was synthesized. The structure of the replicating molecules observed by electron microscopy was mostly sigma shaped, composed of a circle of a unit genome length with a double-stranded tail. These observations suggest that the replication of ColE1 DNA proceeds via a rolling-circle type of structure in the absence of dnaA function.

Links

Publisher Full Text
ncbi.nlm.nih.gov
jb.asm.org
PMC Free PDF

Authors

Abe M
No affiliation info available

MeSH

Centrifugation, Density GradientChloramphenicolDNA ReplicationDNA, BacterialEscherichia coliMutationNucleic Acid ConformationPlasmidsRifampinTemperature

Pub Type(s)

Journal Article

Language

eng

PubMed ID

6988397

Citation

Abe, M. "Replication of ColE1 Plasmid Deoxyribonucleic Acid in a Thermosensitive dnaA Mutant of Escherichia Coli." Journal of Bacteriology, vol. 141, no. 3, 1980, pp. 1024-30.
Abe M. Replication of ColE1 plasmid deoxyribonucleic acid in a thermosensitive dnaA mutant of Escherichia coli. J Bacteriol. 1980;141(3):1024-30.
Abe, M. (1980). Replication of ColE1 plasmid deoxyribonucleic acid in a thermosensitive dnaA mutant of Escherichia coli. Journal of Bacteriology, 141(3), 1024-30.
Abe M. Replication of ColE1 Plasmid Deoxyribonucleic Acid in a Thermosensitive dnaA Mutant of Escherichia Coli. J Bacteriol. 1980;141(3):1024-30. PubMed PMID: 6988397.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Replication of ColE1 plasmid deoxyribonucleic acid in a thermosensitive dnaA mutant of Escherichia coli. A1 - Abe,M, PY - 1980/3/1/pubmed PY - 1980/3/1/medline PY - 1980/3/1/entrez SP - 1024 EP - 30 JF - Journal of bacteriology JO - J Bacteriol VL - 141 IS - 3 N2 - The replication of ColE1 deoxyribonucleic acid (DNA) took place at the restrictive temperature in a dnaA mutant, dnaA167(Ts). It proceeded at a constant rate at 42 degrees C for at least 3 h. The replication was insensitive to rifampin, which blocked replication at the permissive temperature or in the presence of chloramphenicol, even at the restrictive temperature. A linear DNA strand of ColE1 longer than unit genome size was synthesized. The structure of the replicating molecules observed by electron microscopy was mostly sigma shaped, composed of a circle of a unit genome length with a double-stranded tail. These observations suggest that the replication of ColE1 DNA proceeds via a rolling-circle type of structure in the absence of dnaA function. SN - 0021-9193 UR - https://www.unboundmedicine.com/medline/citation/6988397/Replication_of_ColE1_plasmid_deoxyribonucleic_acid_in_a_thermosensitive_dnaA_mutant_of_Escherichia_coli_ L2 - http://jb.asm.org/cgi/pmidlookup?view=long&pmid=6988397 DB - PRIME DP - Unbound Medicine ER -