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Expression of a DNA strand initiation sequence of ColE1 plasmid in a single-stranded DNA phage.
Proc Natl Acad Sci U S A. 1980 Nov; 77(11):6566-70.PN

Abstract

In order to investigate initiation of H-strand (lagging strand) replication of the plasmid ColE1, the origin region fragment (Hae II-E) of ColE1 was inserted into the intergenic region of filamentous DNA phage M13 and cloned. A site capable of promoting DNA strand initiation on a single-stranded DNA template has been detected on the L-strand (leading strand) of the cloned fragment. The site, named rri-1 rifampicin-resistant initiation), directs conversion of chimeric phage single-stranded DNA to parental replicative form in the presence of rifampicin, which blocks the function of the complementary strand origin of M13. The function of rri-1 is dependent on both the dnaG and dnaB gene products. It is postulated that rri-1 might be an initiation site for synthesis of the lagging DNA strand during unidirectional replication of ColE1 DNA.

Authors

No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

7005899

Citation

Nomura, N, and D S. Ray. "Expression of a DNA Strand Initiation Sequence of ColE1 Plasmid in a Single-stranded DNA Phage." Proceedings of the National Academy of Sciences of the United States of America, vol. 77, no. 11, 1980, pp. 6566-70.
Nomura N, Ray DS. Expression of a DNA strand initiation sequence of ColE1 plasmid in a single-stranded DNA phage. Proc Natl Acad Sci USA. 1980;77(11):6566-70.
Nomura, N., & Ray, D. S. (1980). Expression of a DNA strand initiation sequence of ColE1 plasmid in a single-stranded DNA phage. Proceedings of the National Academy of Sciences of the United States of America, 77(11), 6566-70.
Nomura N, Ray DS. Expression of a DNA Strand Initiation Sequence of ColE1 Plasmid in a Single-stranded DNA Phage. Proc Natl Acad Sci USA. 1980;77(11):6566-70. PubMed PMID: 7005899.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of a DNA strand initiation sequence of ColE1 plasmid in a single-stranded DNA phage. AU - Nomura,N, AU - Ray,D S, PY - 1980/11/1/pubmed PY - 1980/11/1/medline PY - 1980/11/1/entrez SP - 6566 EP - 70 JF - Proceedings of the National Academy of Sciences of the United States of America JO - Proc. Natl. Acad. Sci. U.S.A. VL - 77 IS - 11 N2 - In order to investigate initiation of H-strand (lagging strand) replication of the plasmid ColE1, the origin region fragment (Hae II-E) of ColE1 was inserted into the intergenic region of filamentous DNA phage M13 and cloned. A site capable of promoting DNA strand initiation on a single-stranded DNA template has been detected on the L-strand (leading strand) of the cloned fragment. The site, named rri-1 rifampicin-resistant initiation), directs conversion of chimeric phage single-stranded DNA to parental replicative form in the presence of rifampicin, which blocks the function of the complementary strand origin of M13. The function of rri-1 is dependent on both the dnaG and dnaB gene products. It is postulated that rri-1 might be an initiation site for synthesis of the lagging DNA strand during unidirectional replication of ColE1 DNA. SN - 0027-8424 UR - https://www.unboundmedicine.com/medline/citation/7005899/Expression_of_a_DNA_strand_initiation_sequence_of_ColE1_plasmid_in_a_single_stranded_DNA_phage_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/7005899/ DB - PRIME DP - Unbound Medicine ER -