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Inhibition of erythrocyte sickling in vitro by pyridoxal.
J Clin Invest. 1978 Oct; 62(4):888-91.JCI

Abstract

To test the antisickling activity of pyridoxal, we compared the oxygen affinity and the percent sickling at low PO2 of untreated erythrocytes with values for cells from the same blood sample incubated with pyridoxal, glyceraldehyde, or pyridoxine. Pyridoxal increased oxygen affinity much more than glyceraldehyde. 20 mM pyridoxal and glyceraldehyde had equivalent antisickling activity. At PO2 levels above 20 mm Hg, both agents reduced sickling to less than 2%. In samples examined by electron microscopy, pyridoxal reduced the percent sickled cells and the percent cells that contain hemoglobin S fibers by the same amount (from 74 to 3%). Pyridoxine had no effect on oxygen affinity or sockling. Pyridoxal reacts with intracellular hemoglobin to increase oxygen affinity, which inhibits hemoglobin S polymerization and sickling.

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Authors

Kark JA
No affiliation info available
Kale MP
No affiliation info available
Tarassoff PG
No affiliation info available
Woods M
No affiliation info available
Lessin LS
No affiliation info available

MeSH

Anemia, Sickle CellErythrocytes, AbnormalGlyceraldehydeHemoglobin, SickleHumansMacromolecular SubstancesOxygenPyridoxalPyridoxine

Pub Type(s)

Journal Article

Language

eng

PubMed ID

701485

Citation

Kark, J A., et al. "Inhibition of Erythrocyte Sickling in Vitro By Pyridoxal." The Journal of Clinical Investigation, vol. 62, no. 4, 1978, pp. 888-91.
Kark JA, Kale MP, Tarassoff PG, et al. Inhibition of erythrocyte sickling in vitro by pyridoxal. J Clin Invest. 1978;62(4):888-91.
Kark, J. A., Kale, M. P., Tarassoff, P. G., Woods, M., & Lessin, L. S. (1978). Inhibition of erythrocyte sickling in vitro by pyridoxal. The Journal of Clinical Investigation, 62(4), 888-91.
Kark JA, et al. Inhibition of Erythrocyte Sickling in Vitro By Pyridoxal. J Clin Invest. 1978;62(4):888-91. PubMed PMID: 701485.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibition of erythrocyte sickling in vitro by pyridoxal. AU - Kark,J A, AU - Kale,M P, AU - Tarassoff,P G, AU - Woods,M, AU - Lessin,L S, PY - 1978/10/1/pubmed PY - 1978/10/1/medline PY - 1978/10/1/entrez SP - 888 EP - 91 JF - The Journal of clinical investigation JO - J Clin Invest VL - 62 IS - 4 N2 - To test the antisickling activity of pyridoxal, we compared the oxygen affinity and the percent sickling at low PO2 of untreated erythrocytes with values for cells from the same blood sample incubated with pyridoxal, glyceraldehyde, or pyridoxine. Pyridoxal increased oxygen affinity much more than glyceraldehyde. 20 mM pyridoxal and glyceraldehyde had equivalent antisickling activity. At PO2 levels above 20 mm Hg, both agents reduced sickling to less than 2%. In samples examined by electron microscopy, pyridoxal reduced the percent sickled cells and the percent cells that contain hemoglobin S fibers by the same amount (from 74 to 3%). Pyridoxine had no effect on oxygen affinity or sockling. Pyridoxal reacts with intracellular hemoglobin to increase oxygen affinity, which inhibits hemoglobin S polymerization and sickling. SN - 0021-9738 UR - https://www.unboundmedicine.com/medline/citation/701485/Inhibition_of_erythrocyte_sickling_in_vitro_by_pyridoxal_ L2 - https://doi.org/10.1172/JCI109202 DB - PRIME DP - Unbound Medicine ER -