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[Stability and immunogenicity of the fresh BCG vaccine applied with a new multiple puncture device (author's transl)].
Ann Microbiol (Paris) 1981 May-Jun; 132(3):307-18AM

Abstract

A new multiple puncture instrument with interchangeable pieces has been devised. It permits the application of 12 or 28 cutaneous punctures, depending on the age of the patient (child). Fresh concentrated (50 mg/ml) BCG vaccine was distributed either into neutral glass ampoules or into neutral polypropylene tubes. No difference in viability was observed after storage at 4 degrees C and in a series of 10 batches of vaccine, a good survival (about 50%) was found after three months. Also, survival was identical among 9 batches of vaccine, whether it was resuspended in a protective solution containing human albumin plus 5% glycerol or in 6% glycerol only. The immunogenicity of the fresh concentrated vaccine, when applied with the new multiple puncture device, was studied using different tests: --the growth of BCG in organs of mice, --the survival time after virulent challenge of vaccinated versus unvaccinated mice, --tuberculin-delayed hypersensitivity of vaccinated guinea-pigs. The immunogenic potency has been found similar to that observed using other methods of BCG vaccination. These experimental results confirm the merit of the new method of vaccination using the multiple puncture device.

Authors

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Pub Type(s)

English Abstract
Journal Article

Language

fre

PubMed ID

7027870

Citation

Gheorghiu, M, et al. "[Stability and Immunogenicity of the Fresh BCG Vaccine Applied With a New Multiple Puncture Device (author's Transl)]." Annales De Microbiologie, vol. 132, no. 3, 1981, pp. 307-18.
Gheorghiu M, Lagranderie M, Bredèche M. [Stability and immunogenicity of the fresh BCG vaccine applied with a new multiple puncture device (author's transl)]. Ann Microbiol (Paris). 1981;132(3):307-18.
Gheorghiu, M., Lagranderie, M., & Bredèche, M. (1981). [Stability and immunogenicity of the fresh BCG vaccine applied with a new multiple puncture device (author's transl)]. Annales De Microbiologie, 132(3), pp. 307-18.
Gheorghiu M, Lagranderie M, Bredèche M. [Stability and Immunogenicity of the Fresh BCG Vaccine Applied With a New Multiple Puncture Device (author's Transl)]. Ann Microbiol (Paris). 1981;132(3):307-18. PubMed PMID: 7027870.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Stability and immunogenicity of the fresh BCG vaccine applied with a new multiple puncture device (author's transl)]. AU - Gheorghiu,M, AU - Lagranderie,M, AU - Bredèche,M, PY - 1981/5/1/pubmed PY - 1981/5/1/medline PY - 1981/5/1/entrez SP - 307 EP - 18 JF - Annales de microbiologie JO - Ann. Microbiol. (Paris) VL - 132 IS - 3 N2 - A new multiple puncture instrument with interchangeable pieces has been devised. It permits the application of 12 or 28 cutaneous punctures, depending on the age of the patient (child). Fresh concentrated (50 mg/ml) BCG vaccine was distributed either into neutral glass ampoules or into neutral polypropylene tubes. No difference in viability was observed after storage at 4 degrees C and in a series of 10 batches of vaccine, a good survival (about 50%) was found after three months. Also, survival was identical among 9 batches of vaccine, whether it was resuspended in a protective solution containing human albumin plus 5% glycerol or in 6% glycerol only. The immunogenicity of the fresh concentrated vaccine, when applied with the new multiple puncture device, was studied using different tests: --the growth of BCG in organs of mice, --the survival time after virulent challenge of vaccinated versus unvaccinated mice, --tuberculin-delayed hypersensitivity of vaccinated guinea-pigs. The immunogenic potency has been found similar to that observed using other methods of BCG vaccination. These experimental results confirm the merit of the new method of vaccination using the multiple puncture device. SN - 0300-5410 UR - https://www.unboundmedicine.com/medline/citation/7027870/[Stability_and_immunogenicity_of_the_fresh_BCG_vaccine_applied_with_a_new_multiple_puncture_device__author's_transl_]_ L2 - https://medlineplus.gov/immunization.html DB - PRIME DP - Unbound Medicine ER -