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Surface-reactive antibodies to human adrenal cells in Addison's disease.
Clin Exp Immunol. 1981 Jul; 45(1):48-55.CE

Abstract

Organ-specific surface-reactive antibodies to viable human adrenal cell suspensions from adult or fetal glands were detected by indirect immunofluorescence (IFL) in 24 out of 28 idiopathic Addison's disease sera with adrenal cytoplasmic antibodies. Cell-surface reactions were also present in nine out of 10 cases of polyendocrine autoimmune disorders without overt adrenal failure but possessing adrenal cytoplasmic antibodies. None of 18 Addisonian patients, 25 cases with other autoimmune disorders and 10 normal individuals, all negative for adrenal cytoplasmic antibodies, showed positive surface reactions on viable cells. When the surface IFL was done on established monolayers, the positive sera gave variable staining suggesting that more than one antigen may be expressed under different conditions. These results suggest that adrenal-specific 'microsomal' antigens are also represented on the plasma membrane, and support the hypothesis that organ-specific autoantibodies reacting with the surface of living target cells may have a pathogenic role in the development of autoimmune adrenalitis.

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Authors

Khoury EL
No affiliation info available
Hammond L
No affiliation info available
Bottazzo GF
No affiliation info available
Doniach D
No affiliation info available

MeSH

Addison DiseaseAdrenal GlandsAdultAntigens, SurfaceAutoantibodiesAutoantigensCell MembraneCells, CulturedFluorescent Antibody TechniqueHumans

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

7030540

Citation

Khoury, E L., et al. "Surface-reactive Antibodies to Human Adrenal Cells in Addison's Disease." Clinical and Experimental Immunology, vol. 45, no. 1, 1981, pp. 48-55.
Khoury EL, Hammond L, Bottazzo GF, et al. Surface-reactive antibodies to human adrenal cells in Addison's disease. Clin Exp Immunol. 1981;45(1):48-55.
Khoury, E. L., Hammond, L., Bottazzo, G. F., & Doniach, D. (1981). Surface-reactive antibodies to human adrenal cells in Addison's disease. Clinical and Experimental Immunology, 45(1), 48-55.
Khoury EL, et al. Surface-reactive Antibodies to Human Adrenal Cells in Addison's Disease. Clin Exp Immunol. 1981;45(1):48-55. PubMed PMID: 7030540.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Surface-reactive antibodies to human adrenal cells in Addison's disease. AU - Khoury,E L, AU - Hammond,L, AU - Bottazzo,G F, AU - Doniach,D, PY - 1981/7/1/pubmed PY - 1981/7/1/medline PY - 1981/7/1/entrez SP - 48 EP - 55 JF - Clinical and experimental immunology JO - Clin Exp Immunol VL - 45 IS - 1 N2 - Organ-specific surface-reactive antibodies to viable human adrenal cell suspensions from adult or fetal glands were detected by indirect immunofluorescence (IFL) in 24 out of 28 idiopathic Addison's disease sera with adrenal cytoplasmic antibodies. Cell-surface reactions were also present in nine out of 10 cases of polyendocrine autoimmune disorders without overt adrenal failure but possessing adrenal cytoplasmic antibodies. None of 18 Addisonian patients, 25 cases with other autoimmune disorders and 10 normal individuals, all negative for adrenal cytoplasmic antibodies, showed positive surface reactions on viable cells. When the surface IFL was done on established monolayers, the positive sera gave variable staining suggesting that more than one antigen may be expressed under different conditions. These results suggest that adrenal-specific 'microsomal' antigens are also represented on the plasma membrane, and support the hypothesis that organ-specific autoantibodies reacting with the surface of living target cells may have a pathogenic role in the development of autoimmune adrenalitis. SN - 0009-9104 UR - https://www.unboundmedicine.com/medline/citation/7030540/Surface_reactive_antibodies_to_human_adrenal_cells_in_Addison's_disease_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/7030540/ DB - PRIME DP - Unbound Medicine ER -