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Demonstration of vitamin B12 analogues in human sera not detected by microbiological assay.
Br J Haematol. 1982 May; 51(1):171-3.BJ

Abstract

Sera were absorbed with polyacrylamide beads to which purified human intrinsic factor was attached. This procedure removed the vitamin B12 analogues which are measured by microbiological assay with Lactobacillus leichmannii and Euglena gracilis and which are measured in an isotope dilution method using intrinsic factor. Such sera still contained B12 analogues that were assayed in an isotope dilution method using a non-intrinsic factor vitamin B12 binder. Such vitamin B12 analogues make up approximately half of the total vitamin B12 analogues in human serum.

Authors

No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

7041953

Citation

Chanarin, I, and M Muir. "Demonstration of Vitamin B12 Analogues in Human Sera Not Detected By Microbiological Assay." British Journal of Haematology, vol. 51, no. 1, 1982, pp. 171-3.
Chanarin I, Muir M. Demonstration of vitamin B12 analogues in human sera not detected by microbiological assay. Br J Haematol. 1982;51(1):171-3.
Chanarin, I., & Muir, M. (1982). Demonstration of vitamin B12 analogues in human sera not detected by microbiological assay. British Journal of Haematology, 51(1), 171-3.
Chanarin I, Muir M. Demonstration of Vitamin B12 Analogues in Human Sera Not Detected By Microbiological Assay. Br J Haematol. 1982;51(1):171-3. PubMed PMID: 7041953.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Demonstration of vitamin B12 analogues in human sera not detected by microbiological assay. AU - Chanarin,I, AU - Muir,M, PY - 1982/5/1/pubmed PY - 1982/5/1/medline PY - 1982/5/1/entrez SP - 171 EP - 3 JF - British journal of haematology JO - Br J Haematol VL - 51 IS - 1 N2 - Sera were absorbed with polyacrylamide beads to which purified human intrinsic factor was attached. This procedure removed the vitamin B12 analogues which are measured by microbiological assay with Lactobacillus leichmannii and Euglena gracilis and which are measured in an isotope dilution method using intrinsic factor. Such sera still contained B12 analogues that were assayed in an isotope dilution method using a non-intrinsic factor vitamin B12 binder. Such vitamin B12 analogues make up approximately half of the total vitamin B12 analogues in human serum. SN - 0007-1048 UR - https://www.unboundmedicine.com/medline/citation/7041953/Demonstration_of_vitamin_B12_analogues_in_human_sera_not_detected_by_microbiological_assay_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0007-1048&date=1982&volume=51&issue=1&spage=171 DB - PRIME DP - Unbound Medicine ER -