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Improved diagnostic medium for separation of Cryptococcus neoformans var. neoformans (serotypes A and D) and Cryptococcus neoformans var. gattii (serotypes B and C).
J Clin Microbiol. 1982 Mar; 15(3):535-7.JC

Abstract

A simple new agar medium containing L-canavanine, glycine, and bromthymol blue was found to give a clearer and more accurate distinction between serotype A or D (Cryptococcus neoformans var. neoformans) and serotype B or C (C. neoformans var. gattii) than creatinine-dextrose-bromthymol blue or glycine-cycloheximide-phenol red media. Among 143 isolates of serotype A or D and 70 isolates of serotype B or C, the new medium correlated completely with the serotype, whereas nearly 11% of these isolates gave discrepant reactions with creatinine-dextrose-bromthymol blue and glycine-cycloheximide-phenol red media.

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Authors

Kwon-Chung KJ
No affiliation info available
Polacheck I
No affiliation info available
Bennett JE
No affiliation info available

MeSH

CryptococcusCryptococcus neoformansCulture MediaSerotyping

Pub Type(s)

Journal Article

Language

eng

PubMed ID

7042750

Citation

Kwon-Chung, K J., et al. "Improved Diagnostic Medium for Separation of Cryptococcus Neoformans Var. Neoformans (serotypes a and D) and Cryptococcus Neoformans Var. Gattii (serotypes B and C)." Journal of Clinical Microbiology, vol. 15, no. 3, 1982, pp. 535-7.
Kwon-Chung KJ, Polacheck I, Bennett JE. Improved diagnostic medium for separation of Cryptococcus neoformans var. neoformans (serotypes A and D) and Cryptococcus neoformans var. gattii (serotypes B and C). J Clin Microbiol. 1982;15(3):535-7.
Kwon-Chung, K. J., Polacheck, I., & Bennett, J. E. (1982). Improved diagnostic medium for separation of Cryptococcus neoformans var. neoformans (serotypes A and D) and Cryptococcus neoformans var. gattii (serotypes B and C). Journal of Clinical Microbiology, 15(3), 535-7.
Kwon-Chung KJ, Polacheck I, Bennett JE. Improved Diagnostic Medium for Separation of Cryptococcus Neoformans Var. Neoformans (serotypes a and D) and Cryptococcus Neoformans Var. Gattii (serotypes B and C). J Clin Microbiol. 1982;15(3):535-7. PubMed PMID: 7042750.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Improved diagnostic medium for separation of Cryptococcus neoformans var. neoformans (serotypes A and D) and Cryptococcus neoformans var. gattii (serotypes B and C). AU - Kwon-Chung,K J, AU - Polacheck,I, AU - Bennett,J E, PY - 1982/3/1/pubmed PY - 1982/3/1/medline PY - 1982/3/1/entrez SP - 535 EP - 7 JF - Journal of clinical microbiology JO - J Clin Microbiol VL - 15 IS - 3 N2 - A simple new agar medium containing L-canavanine, glycine, and bromthymol blue was found to give a clearer and more accurate distinction between serotype A or D (Cryptococcus neoformans var. neoformans) and serotype B or C (C. neoformans var. gattii) than creatinine-dextrose-bromthymol blue or glycine-cycloheximide-phenol red media. Among 143 isolates of serotype A or D and 70 isolates of serotype B or C, the new medium correlated completely with the serotype, whereas nearly 11% of these isolates gave discrepant reactions with creatinine-dextrose-bromthymol blue and glycine-cycloheximide-phenol red media. SN - 0095-1137 UR - https://www.unboundmedicine.com/medline/citation/7042750/Improved_diagnostic_medium_for_separation_of_Cryptococcus_neoformans_var__neoformans__serotypes_A_and_D__and_Cryptococcus_neoformans_var__gattii__serotypes_B_and_C__ L2 - https://journals.asm.org/doi/10.1128/jcm.15.3.535-537.1982?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -