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Plasmid ColE1 conjugal mobility: the nature of bom, a region required in cis for transfer.
Mol Gen Genet. 1982; 185(2):344-51.MG

Abstract

Conjugal mobility of ColE1 and related plasmids is promoted by a wide range of conjugative plasmids. ColE1 produces trans-acting products and has a region required in cis (bom ; basis of mobility) for such mobility. Here we show that plasmid pBR322 contains a functional bom sequence located within a 141 bp HhaI fragment. This bom region is functional for conjugation promoted by several different conjugative plasmids and is highly conserved in ColE1 and contains nic the putative origin of transfer. The orientation and position of bom with respect to the ColE1 vegetative origin of replication can be changed without affecting the frequency of conjugal mobility promoted by R64drd11.

Authors

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Pub Type(s)

Journal Article

Language

eng

PubMed ID

7045589

Citation

Finnegan, J, and D Sherratt. "Plasmid ColE1 Conjugal Mobility: the Nature of Bom, a Region Required in Cis for Transfer." Molecular & General Genetics : MGG, vol. 185, no. 2, 1982, pp. 344-51.
Finnegan J, Sherratt D. Plasmid ColE1 conjugal mobility: the nature of bom, a region required in cis for transfer. Mol Gen Genet. 1982;185(2):344-51.
Finnegan, J., & Sherratt, D. (1982). Plasmid ColE1 conjugal mobility: the nature of bom, a region required in cis for transfer. Molecular & General Genetics : MGG, 185(2), 344-51.
Finnegan J, Sherratt D. Plasmid ColE1 Conjugal Mobility: the Nature of Bom, a Region Required in Cis for Transfer. Mol Gen Genet. 1982;185(2):344-51. PubMed PMID: 7045589.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Plasmid ColE1 conjugal mobility: the nature of bom, a region required in cis for transfer. AU - Finnegan,J, AU - Sherratt,D, PY - 1982/1/1/pubmed PY - 1982/1/1/medline PY - 1982/1/1/entrez SP - 344 EP - 51 JF - Molecular & general genetics : MGG JO - Mol. Gen. Genet. VL - 185 IS - 2 N2 - Conjugal mobility of ColE1 and related plasmids is promoted by a wide range of conjugative plasmids. ColE1 produces trans-acting products and has a region required in cis (bom ; basis of mobility) for such mobility. Here we show that plasmid pBR322 contains a functional bom sequence located within a 141 bp HhaI fragment. This bom region is functional for conjugation promoted by several different conjugative plasmids and is highly conserved in ColE1 and contains nic the putative origin of transfer. The orientation and position of bom with respect to the ColE1 vegetative origin of replication can be changed without affecting the frequency of conjugal mobility promoted by R64drd11. SN - 0026-8925 UR - https://www.unboundmedicine.com/medline/citation/7045589/Plasmid_ColE1_conjugal_mobility:_the_nature_of_bom_a_region_required_in_cis_for_transfer_ L2 - https://doi.org/10.1007/BF00330810 DB - PRIME DP - Unbound Medicine ER -