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Methemoglobin in blood as determined by double-wavelength spectrophotometry.
Clin Chem. 1982 Mar; 28(3):508-11.CC

Abstract

We describe spectrophotometric determination of methemoglobin (Met-Hb) in blood by absorbance differences at two wavelengths (characteristic of the instrument used) in the 500-600 nm region. Blood is diluted 100- to 200-fold with a solution containing KCN and carbon monoxide to convert hemoglobin to carboxyhemoglobin and Met-Hb to cyanomethemoglobin (CN-Met-Hb). Carboxyhemoglobin has the same absorbance at the two wavelengths used, so that the differences in absorbance reflect only the CN-Met-Hb component. After measurement of absorbance differences, potassium ferricyanide is added to convert all hemoglobin derivatives to CN-Met-Hb; samples are then remeasured at the same wavelengths. The percentage of Met-Hb is determined from the ratio of the absorbance differences. Results agree satisfactorily with currently accepted procedures and with calculated theoretical results for mixtures of oxyhemoglobin, carboxyhemoglobin, and Met-Hb.

Authors

Sakata M
No affiliation info available
Yoshida A
No affiliation info available
Haga M
No affiliation info available

MeSH

CarboxyhemoglobinHumansMethemoglobinSpectrophotometrySulfhemoglobin

Pub Type(s)

Journal Article

Language

eng

PubMed ID

7067094

Citation

Sakata, M, et al. "Methemoglobin in Blood as Determined By Double-wavelength Spectrophotometry." Clinical Chemistry, vol. 28, no. 3, 1982, pp. 508-11.
Sakata M, Yoshida A, Haga M. Methemoglobin in blood as determined by double-wavelength spectrophotometry. Clin Chem. 1982;28(3):508-11.
Sakata, M., Yoshida, A., & Haga, M. (1982). Methemoglobin in blood as determined by double-wavelength spectrophotometry. Clinical Chemistry, 28(3), 508-11.
Sakata M, Yoshida A, Haga M. Methemoglobin in Blood as Determined By Double-wavelength Spectrophotometry. Clin Chem. 1982;28(3):508-11. PubMed PMID: 7067094.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Methemoglobin in blood as determined by double-wavelength spectrophotometry. AU - Sakata,M, AU - Yoshida,A, AU - Haga,M, PY - 1982/3/1/pubmed PY - 1982/3/1/medline PY - 1982/3/1/entrez SP - 508 EP - 11 JF - Clinical chemistry JO - Clin Chem VL - 28 IS - 3 N2 - We describe spectrophotometric determination of methemoglobin (Met-Hb) in blood by absorbance differences at two wavelengths (characteristic of the instrument used) in the 500-600 nm region. Blood is diluted 100- to 200-fold with a solution containing KCN and carbon monoxide to convert hemoglobin to carboxyhemoglobin and Met-Hb to cyanomethemoglobin (CN-Met-Hb). Carboxyhemoglobin has the same absorbance at the two wavelengths used, so that the differences in absorbance reflect only the CN-Met-Hb component. After measurement of absorbance differences, potassium ferricyanide is added to convert all hemoglobin derivatives to CN-Met-Hb; samples are then remeasured at the same wavelengths. The percentage of Met-Hb is determined from the ratio of the absorbance differences. Results agree satisfactorily with currently accepted procedures and with calculated theoretical results for mixtures of oxyhemoglobin, carboxyhemoglobin, and Met-Hb. SN - 0009-9147 UR - https://www.unboundmedicine.com/medline/citation/7067094/Methemoglobin_in_blood_as_determined_by_double_wavelength_spectrophotometry_ L2 - https://antibodies.cancer.gov/detail/CPTC-MET-1 DB - PRIME DP - Unbound Medicine ER -