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Amino acid profiles in inner ear fluids and cerebrospinal fluid.
Laryngoscope. 1982 Mar; 92(3):321-8.L

Abstract

The levels of 19 amino acids in utricular endolymph, vestibular and cochlear perilymph, and cerebrospinal fluid of guinea pigs were determined using gradient elution reverse phase high performance liquid chromatography of the o-phthaldialdehyde-ethanethiol adducts with fluorescence detection. Aspartate and glutamate were significantly higher in endolymph than in perilymph, in agreement with earlier results on cochlear fluids based on enzymatic fluorometric techniques. All other amino acids tested were significantly lower in the endolymph, in most cases by an order of magnitude. Vestibular perilymph and perilymph of scala vestibuli are virtually identical. Amino acid levels were all higher in perilymph of scala vestibuli than in cerebrospinal fluid; two by an order of magnitude. All differences were statistically significant, with the exception of aspartate. Amino acid levels in perilymph of scala tympani were highly variable dependent upon sampling technique, and no definite values are therefore presented. Comparisons with results from other laboratories, technical pitfalls, and possible implications and interpretations of the results are presented.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

7070173

Citation

Thalmann, R, et al. "Amino Acid Profiles in Inner Ear Fluids and Cerebrospinal Fluid." The Laryngoscope, vol. 92, no. 3, 1982, pp. 321-8.
Thalmann R, Comegys TH, Thalmann I. Amino acid profiles in inner ear fluids and cerebrospinal fluid. Laryngoscope. 1982;92(3):321-8.
Thalmann, R., Comegys, T. H., & Thalmann, I. (1982). Amino acid profiles in inner ear fluids and cerebrospinal fluid. The Laryngoscope, 92(3), 321-8.
Thalmann R, Comegys TH, Thalmann I. Amino Acid Profiles in Inner Ear Fluids and Cerebrospinal Fluid. Laryngoscope. 1982;92(3):321-8. PubMed PMID: 7070173.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Amino acid profiles in inner ear fluids and cerebrospinal fluid. AU - Thalmann,R, AU - Comegys,T H, AU - Thalmann,I, PY - 1982/3/1/pubmed PY - 1982/3/1/medline PY - 1982/3/1/entrez SP - 321 EP - 8 JF - The Laryngoscope JO - Laryngoscope VL - 92 IS - 3 N2 - The levels of 19 amino acids in utricular endolymph, vestibular and cochlear perilymph, and cerebrospinal fluid of guinea pigs were determined using gradient elution reverse phase high performance liquid chromatography of the o-phthaldialdehyde-ethanethiol adducts with fluorescence detection. Aspartate and glutamate were significantly higher in endolymph than in perilymph, in agreement with earlier results on cochlear fluids based on enzymatic fluorometric techniques. All other amino acids tested were significantly lower in the endolymph, in most cases by an order of magnitude. Vestibular perilymph and perilymph of scala vestibuli are virtually identical. Amino acid levels were all higher in perilymph of scala vestibuli than in cerebrospinal fluid; two by an order of magnitude. All differences were statistically significant, with the exception of aspartate. Amino acid levels in perilymph of scala tympani were highly variable dependent upon sampling technique, and no definite values are therefore presented. Comparisons with results from other laboratories, technical pitfalls, and possible implications and interpretations of the results are presented. SN - 0023-852X UR - https://www.unboundmedicine.com/medline/citation/7070173/Amino_acid_profiles_in_inner_ear_fluids_and_cerebrospinal_fluid_ L2 - https://doi.org/10.1288/00005537-198203000-00018 DB - PRIME DP - Unbound Medicine ER -