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Detection of measles IgM antibodies by enzyme-linked immunosorbent assay (ELISA).
Acta Pathol Microbiol Immunol Scand B. 1982 Apr; 90(2):153-60.AP

Abstract

An enzyme-linked immunosorbent assay (ELISA) for the detectors of IgM antibodies in serum during acute or recent measles infections is described. False positive reactions due to rheumatoid factors (RF) were not observed in the test of human sera to which were added sera containing RF. Monospecific antisera against the haemagglutinin, nucleoprotein and polymerase of measles virus were used in a blocking IgM ELISA, revealing a sensitive method for the detection of the antibodies that take part in the immune reactions. An ELISA for the detection of measles-specific IgG in order to reveal IgM or IgG antibodies in the human sera is also described.

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Publisher Full Text

Authors

Pedersen IR
No affiliation info available
Antonsdottir A
No affiliation info available
Evald T
No affiliation info available
Mordhorst CH
No affiliation info available

MeSH

AnimalsAntibodies, ViralComplement Fixation TestsCross ReactionsEnzyme-Linked Immunosorbent AssayFalse Positive ReactionsHumansImmunoglobulin GImmunoglobulin MMeaslesMeasles virusRabbitsRheumatoid Factor

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

7080832

Citation

Pedersen, I R., et al. "Detection of Measles IgM Antibodies By Enzyme-linked Immunosorbent Assay (ELISA)." Acta Pathologica, Microbiologica, Et Immunologica Scandinavica. Section B, Microbiology, vol. 90, no. 2, 1982, pp. 153-60.
Pedersen IR, Antonsdottir A, Evald T, et al. Detection of measles IgM antibodies by enzyme-linked immunosorbent assay (ELISA). Acta Pathol Microbiol Immunol Scand B. 1982;90(2):153-60.
Pedersen, I. R., Antonsdottir, A., Evald, T., & Mordhorst, C. H. (1982). Detection of measles IgM antibodies by enzyme-linked immunosorbent assay (ELISA). Acta Pathologica, Microbiologica, Et Immunologica Scandinavica. Section B, Microbiology, 90(2), 153-60.
Pedersen IR, et al. Detection of Measles IgM Antibodies By Enzyme-linked Immunosorbent Assay (ELISA). Acta Pathol Microbiol Immunol Scand B. 1982;90(2):153-60. PubMed PMID: 7080832.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of measles IgM antibodies by enzyme-linked immunosorbent assay (ELISA). AU - Pedersen,I R, AU - Antonsdottir,A, AU - Evald,T, AU - Mordhorst,C H, PY - 1982/4/1/pubmed PY - 1982/4/1/medline PY - 1982/4/1/entrez SP - 153 EP - 60 JF - Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology JO - Acta Pathol Microbiol Immunol Scand B VL - 90 IS - 2 N2 - An enzyme-linked immunosorbent assay (ELISA) for the detectors of IgM antibodies in serum during acute or recent measles infections is described. False positive reactions due to rheumatoid factors (RF) were not observed in the test of human sera to which were added sera containing RF. Monospecific antisera against the haemagglutinin, nucleoprotein and polymerase of measles virus were used in a blocking IgM ELISA, revealing a sensitive method for the detection of the antibodies that take part in the immune reactions. An ELISA for the detection of measles-specific IgG in order to reveal IgM or IgG antibodies in the human sera is also described. SN - 0108-0180 UR - https://www.unboundmedicine.com/medline/citation/7080832/Detection_of_measles_IgM_antibodies_by_enzyme_linked_immunosorbent_assay__ELISA__ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0108-0180&date=1982&volume=90&issue=2&spage=153 DB - PRIME DP - Unbound Medicine ER -