TY - JOUR T1 - Graphite furnace atomic absorption spectrophotometric determination of 4-hydroxy-3-nitrobenzenearsonic acid, other organic arsenicals, and inorganic arsenic in finished animal feed. AU - George,G M, AU - Frahm,L J, AU - McDonnell,J P, PY - 1982/5/1/pubmed PY - 1982/5/1/medline PY - 1982/5/1/entrez SP - 711 EP - 9 JF - Journal - Association of Official Analytical Chemists JO - J Assoc Off Anal Chem VL - 65 IS - 3 N2 - 4-Hydroxy-3-nitrobenzenearsonic acid (roxarsone) is administered in animal feed as a growth stimulant over a concentration range of 25-50 ppm. The drug is extracted from 5 g feed with 200 mL aqueous 1.0% ammonium carbonate solution and 5 min of mechanical shaking. Undissolved feed particles are allowed to settle and 1.0 mL aliquot of extract is diluted with 9.0 mL 15% methanol solution. This solution is subjected to sample atomization by a graphite furnace and arsenic detection by atomic absorption spectrophotometry (AAS). Roxarsone recovery from nonmedicated commercial feed fortified at 25 ppm was 103.6% with a relative standard deviation (RSD) of 4.0%. Recovery for 50 ppm fortification was 104.5% (RSD 4.3%). Roxarsone assay results by furnace AAS were compared with results by the current AOAC spectrophotometric method and the AOAC total arsenic method. Results by the 3 methods compare well. The procedure was also used to determine other organic arsenicals and inorganic arsenic in laboratory-fortified feed samples; these recoveries were essentially theoretical. SN - 0004-5756 UR - https://www.unboundmedicine.com/medline/citation/7096254/Graphite_furnace_atomic_absorption_spectrophotometric_determination_of_4_hydroxy_3_nitrobenzenearsonic_acid_other_organic_arsenicals_and_inorganic_arsenic_in_finished_animal_feed_ L2 - https://medlineplus.gov/arsenic.html DB - PRIME DP - Unbound Medicine ER -