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Effects of red cell membrane on the polymerization of sickle hemoglobin.
Blood Cells. 1982; 8(2):237-43.BC

Abstract

We have investigated the effect of erythrocyte membrane preparations on the rate of polymerization and the solubility of deoxyhemoglobin S. The kinetics of polymerization were monitored by measuring changes in turbidity of hemoglobin S solutions as a function of time in a temperature-controlled cuvette. The solubility of deoxyhemoglobin S, an equilibrium measurement, was determined by measuring the hemoglobin concentration of the supernatant following centrifugation of the polymerized hemoglobin S solution. The solubility of deoxyhemoglobin S was unaffected by the presence of red cell membrane ghosts, inside-out red cell vesicles, or inside-out vesicles enriched in erythrocyte spectrin and actin (Bands 1, 2, and 5) in the hemoglobin solution. Furthermore, inside-out vesicles had no significant effect on the kinetics of polymerization. The addition of erythrocyte membrane ghosts to the hemoglobin solution, in ratios approximating that of the intact red cell, increased the rate of polymerization up to 8-fold. However, when corrections were made for the excluded volume contributed by the membrane ghosts, the enhancement of the rate of polymerization was reduced to less than 3-fold. These results fail to show significant facilitation of intracellular sickling by nucleation sites on the red cell membrane.

Authors

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Pub Type(s)

Journal Article

Language

eng

PubMed ID

7159748

Citation

Goldberg, M A., et al. "Effects of Red Cell Membrane On the Polymerization of Sickle Hemoglobin." Blood Cells, vol. 8, no. 2, 1982, pp. 237-43.
Goldberg MA, Lalos AT, Himmelstein B, et al. Effects of red cell membrane on the polymerization of sickle hemoglobin. Blood Cells. 1982;8(2):237-43.
Goldberg, M. A., Lalos, A. T., Himmelstein, B., & Bunn, H. F. (1982). Effects of red cell membrane on the polymerization of sickle hemoglobin. Blood Cells, 8(2), 237-43.
Goldberg MA, et al. Effects of Red Cell Membrane On the Polymerization of Sickle Hemoglobin. Blood Cells. 1982;8(2):237-43. PubMed PMID: 7159748.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of red cell membrane on the polymerization of sickle hemoglobin. AU - Goldberg,M A, AU - Lalos,A T, AU - Himmelstein,B, AU - Bunn,H F, PY - 1982/1/1/pubmed PY - 1982/1/1/medline PY - 1982/1/1/entrez SP - 237 EP - 43 JF - Blood cells JO - Blood Cells VL - 8 IS - 2 N2 - We have investigated the effect of erythrocyte membrane preparations on the rate of polymerization and the solubility of deoxyhemoglobin S. The kinetics of polymerization were monitored by measuring changes in turbidity of hemoglobin S solutions as a function of time in a temperature-controlled cuvette. The solubility of deoxyhemoglobin S, an equilibrium measurement, was determined by measuring the hemoglobin concentration of the supernatant following centrifugation of the polymerized hemoglobin S solution. The solubility of deoxyhemoglobin S was unaffected by the presence of red cell membrane ghosts, inside-out red cell vesicles, or inside-out vesicles enriched in erythrocyte spectrin and actin (Bands 1, 2, and 5) in the hemoglobin solution. Furthermore, inside-out vesicles had no significant effect on the kinetics of polymerization. The addition of erythrocyte membrane ghosts to the hemoglobin solution, in ratios approximating that of the intact red cell, increased the rate of polymerization up to 8-fold. However, when corrections were made for the excluded volume contributed by the membrane ghosts, the enhancement of the rate of polymerization was reduced to less than 3-fold. These results fail to show significant facilitation of intracellular sickling by nucleation sites on the red cell membrane. SN - 0340-4684 UR - https://www.unboundmedicine.com/medline/citation/7159748/Effects_of_red_cell_membrane_on_the_polymerization_of_sickle_hemoglobin_ DB - PRIME DP - Unbound Medicine ER -