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Sickling as a function of oxygen delivery: effect of simulated transfusions of stored, fresh and inositol-hexaphosphate-loaded (low affinity) red cells.
Blood Cells. 1982; 8(2):263-72.BC

Abstract

Low oxygen affinity red cells were prepared by incorporating inositol hexaphosphate (IHP) into red blood cells (rbc) by means of a liposomal transport system. The effect of in vitro simulated exchange transfusions on sickling was studied with buffered red cell suspensions containing 50% SS cells and 50% test cells. Test rbc were either stored cells with high oxygen affinity, fresh cells with normal affinity or IHP-loaded cells with decreased affinity. Oxygen equilibrium curves and percentage sickling as a function of PO2 were determined and the data analyzed in terms of percentage sickling as a function of oxygen delivery. Our simplified analysis shows that simulated exchange transfusion with stored and, to a lesser extent even with fresh blood, results in a decreased venous PO2 and increased sickling of the remaining SS cells. In contrast, transfusion with IHP-loaded cells results in higher venous PO2 values and less sickling throughout the range of oxygen delivery. Thus, the transfusion of IHP-loaded cells may result in less sickling of the remaining SS cells in addition to the normal dilutional effect.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

7159750

Citation

Kumpati, J, et al. "Sickling as a Function of Oxygen Delivery: Effect of Simulated Transfusions of Stored, Fresh and Inositol-hexaphosphate-loaded (low Affinity) Red Cells." Blood Cells, vol. 8, no. 2, 1982, pp. 263-72.
Kumpati J, Franco RS, Weiner M, et al. Sickling as a function of oxygen delivery: effect of simulated transfusions of stored, fresh and inositol-hexaphosphate-loaded (low affinity) red cells. Blood Cells. 1982;8(2):263-72.
Kumpati, J., Franco, R. S., Weiner, M., & Martelo, O. J. (1982). Sickling as a function of oxygen delivery: effect of simulated transfusions of stored, fresh and inositol-hexaphosphate-loaded (low affinity) red cells. Blood Cells, 8(2), 263-72.
Kumpati J, et al. Sickling as a Function of Oxygen Delivery: Effect of Simulated Transfusions of Stored, Fresh and Inositol-hexaphosphate-loaded (low Affinity) Red Cells. Blood Cells. 1982;8(2):263-72. PubMed PMID: 7159750.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sickling as a function of oxygen delivery: effect of simulated transfusions of stored, fresh and inositol-hexaphosphate-loaded (low affinity) red cells. AU - Kumpati,J, AU - Franco,R S, AU - Weiner,M, AU - Martelo,O J, PY - 1982/1/1/pubmed PY - 1982/1/1/medline PY - 1982/1/1/entrez SP - 263 EP - 72 JF - Blood cells JO - Blood Cells VL - 8 IS - 2 N2 - Low oxygen affinity red cells were prepared by incorporating inositol hexaphosphate (IHP) into red blood cells (rbc) by means of a liposomal transport system. The effect of in vitro simulated exchange transfusions on sickling was studied with buffered red cell suspensions containing 50% SS cells and 50% test cells. Test rbc were either stored cells with high oxygen affinity, fresh cells with normal affinity or IHP-loaded cells with decreased affinity. Oxygen equilibrium curves and percentage sickling as a function of PO2 were determined and the data analyzed in terms of percentage sickling as a function of oxygen delivery. Our simplified analysis shows that simulated exchange transfusion with stored and, to a lesser extent even with fresh blood, results in a decreased venous PO2 and increased sickling of the remaining SS cells. In contrast, transfusion with IHP-loaded cells results in higher venous PO2 values and less sickling throughout the range of oxygen delivery. Thus, the transfusion of IHP-loaded cells may result in less sickling of the remaining SS cells in addition to the normal dilutional effect. SN - 0340-4684 UR - https://www.unboundmedicine.com/medline/citation/7159750/Sickling_as_a_function_of_oxygen_delivery:_effect_of_simulated_transfusions_of_stored_fresh_and_inositol_hexaphosphate_loaded__low_affinity__red_cells_ DB - PRIME DP - Unbound Medicine ER -