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A simple spectrophotometry for determination of carboxyhemoglobin in blood.
J Forensic Sci. 1982 Oct; 27(4):928-34.JF

Abstract

A simple spectrophotometric method for determination of carboxyhemoglobin (HbCO) in blood is described. Blood is dissolved in sodium carbonate solution at a dilution near 250-fold. The diluent contains sodium hydrosulfite providing the two-component system HbCO- reduced hemoglobin (HHb). The percentage of HbCO is calculated from absorbance values at 532 and 558 nm measured after the addition of sodium hydroxide, which makes the solution completely clear. Results obtained by the present method are in satisfactory agreement with those by the oxygen electrode method.

Authors

Katsumata Y
No affiliation info available
Aoki M
No affiliation info available
Sato K
No affiliation info available
Suzuki O
No affiliation info available
Oya M
No affiliation info available
Yada S
No affiliation info available

MeSH

CarboxyhemoglobinHemoglobinsHumansSpectrophotometry

Pub Type(s)

Journal Article

Language

eng

PubMed ID

7175473

Citation

Katsumata, Y, et al. "A Simple Spectrophotometry for Determination of Carboxyhemoglobin in Blood." Journal of Forensic Sciences, vol. 27, no. 4, 1982, pp. 928-34.
Katsumata Y, Aoki M, Sato K, et al. A simple spectrophotometry for determination of carboxyhemoglobin in blood. J Forensic Sci. 1982;27(4):928-34.
Katsumata, Y., Aoki, M., Sato, K., Suzuki, O., Oya, M., & Yada, S. (1982). A simple spectrophotometry for determination of carboxyhemoglobin in blood. Journal of Forensic Sciences, 27(4), 928-34.
Katsumata Y, et al. A Simple Spectrophotometry for Determination of Carboxyhemoglobin in Blood. J Forensic Sci. 1982;27(4):928-34. PubMed PMID: 7175473.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A simple spectrophotometry for determination of carboxyhemoglobin in blood. AU - Katsumata,Y, AU - Aoki,M, AU - Sato,K, AU - Suzuki,O, AU - Oya,M, AU - Yada,S, PY - 1982/10/1/pubmed PY - 1982/10/1/medline PY - 1982/10/1/entrez SP - 928 EP - 34 JF - Journal of forensic sciences JO - J Forensic Sci VL - 27 IS - 4 N2 - A simple spectrophotometric method for determination of carboxyhemoglobin (HbCO) in blood is described. Blood is dissolved in sodium carbonate solution at a dilution near 250-fold. The diluent contains sodium hydrosulfite providing the two-component system HbCO- reduced hemoglobin (HHb). The percentage of HbCO is calculated from absorbance values at 532 and 558 nm measured after the addition of sodium hydroxide, which makes the solution completely clear. Results obtained by the present method are in satisfactory agreement with those by the oxygen electrode method. SN - 0022-1198 UR - https://www.unboundmedicine.com/medline/citation/7175473/A_simple_spectrophotometry_for_determination_of_carboxyhemoglobin_in_blood_ DB - PRIME DP - Unbound Medicine ER -