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The single erythrocyte rigidometer (SER) as a reference for RBC deformability.
Biorheology 1982; 19(6):737-53B

Abstract

The SER allows the "deformability" of individual red blood cells to be quantitated by determining their passage time through a pore (d = 5.8 microns, 1 = 50 microns) under the shear stresses of 1.5 Pa-4 Pa. Using this system, we examined the influence of: 1. cytoplasmic viscosity, 2. membrane viscoelastic properties, 3. area to volume relationship. To change these determinants of RBC-deformability, the cells have been altered with 1. Acetylphenylhydrazine (0.016 mol/l), 2. diamide (0.5 mmol/l), 3. osmotic swelling (200 mosm/l) and osmotic shrinking (480 mosm/l) by suspending the cells in hypo- and hypertonic saline. The passage time has been found to be primarily influenced by changes in cytoplasmic viscosity. The same cells when tested in 4 other systems considered to measure RBC-deformability (filtrometer, packed cell viscometry, rheoscope and ektacytometry) behaved differently.

Authors

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Pub Type(s)

Journal Article

Language

eng

PubMed ID

7184522

Citation

Kiesewetter, H, et al. "The Single Erythrocyte Rigidometer (SER) as a Reference for RBC Deformability." Biorheology, vol. 19, no. 6, 1982, pp. 737-53.
Kiesewetter H, Dauer U, Teitel P, et al. The single erythrocyte rigidometer (SER) as a reference for RBC deformability. Biorheology. 1982;19(6):737-53.
Kiesewetter, H., Dauer, U., Teitel, P., Schmid-Schönbein, H., & Trapp, R. (1982). The single erythrocyte rigidometer (SER) as a reference for RBC deformability. Biorheology, 19(6), pp. 737-53.
Kiesewetter H, et al. The Single Erythrocyte Rigidometer (SER) as a Reference for RBC Deformability. Biorheology. 1982;19(6):737-53. PubMed PMID: 7184522.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The single erythrocyte rigidometer (SER) as a reference for RBC deformability. AU - Kiesewetter,H, AU - Dauer,U, AU - Teitel,P, AU - Schmid-Schönbein,H, AU - Trapp,R, PY - 1982/1/1/pubmed PY - 1982/1/1/medline PY - 1982/1/1/entrez SP - 737 EP - 53 JF - Biorheology JO - Biorheology VL - 19 IS - 6 N2 - The SER allows the "deformability" of individual red blood cells to be quantitated by determining their passage time through a pore (d = 5.8 microns, 1 = 50 microns) under the shear stresses of 1.5 Pa-4 Pa. Using this system, we examined the influence of: 1. cytoplasmic viscosity, 2. membrane viscoelastic properties, 3. area to volume relationship. To change these determinants of RBC-deformability, the cells have been altered with 1. Acetylphenylhydrazine (0.016 mol/l), 2. diamide (0.5 mmol/l), 3. osmotic swelling (200 mosm/l) and osmotic shrinking (480 mosm/l) by suspending the cells in hypo- and hypertonic saline. The passage time has been found to be primarily influenced by changes in cytoplasmic viscosity. The same cells when tested in 4 other systems considered to measure RBC-deformability (filtrometer, packed cell viscometry, rheoscope and ektacytometry) behaved differently. SN - 0006-355X UR - https://www.unboundmedicine.com/medline/citation/7184522/The_single_erythrocyte_rigidometer__SER__as_a_reference_for_RBC_deformability_ DB - PRIME DP - Unbound Medicine ER -