Tags

Type your tag names separated by a space and hit enter

Competitive binding studies of compounds that interact with DNA utilizing fluorescence polarization.
Biochim Biophys Acta. 1981 Jan 29; 652(1):55-63.BB

Abstract

The increase in the fluorescence polarization of acridine orange upon binding to DNA molecules is used as the basis of a competitive method to study the interaction of a variety of fluorescent and non-fluorescent compounds with DNA. Test compounds that interact with DNA inhibit both the binding of acridine orange to DNA and the accompanying increase in fluorescence polarization. Actinomycin D exhibits a dose-dependent inhibition of acridine orange-DNA binding with Micrococcus luteus DNA, calf thymus DNA, and poly(dG-dC); no detectable inhibition of acridine orange intercalation into poly(dA-dT) is observed. In contrast, proflavine shows similar acridine orange inhibition for poly(dA-dT), calf thymus DNA and M. luteus DNA.

Authors

No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

7213736

Citation

Richardson, C L., and G E. Schulman. "Competitive Binding Studies of Compounds That Interact With DNA Utilizing Fluorescence Polarization." Biochimica Et Biophysica Acta, vol. 652, no. 1, 1981, pp. 55-63.
Richardson CL, Schulman GE. Competitive binding studies of compounds that interact with DNA utilizing fluorescence polarization. Biochim Biophys Acta. 1981;652(1):55-63.
Richardson, C. L., & Schulman, G. E. (1981). Competitive binding studies of compounds that interact with DNA utilizing fluorescence polarization. Biochimica Et Biophysica Acta, 652(1), 55-63.
Richardson CL, Schulman GE. Competitive Binding Studies of Compounds That Interact With DNA Utilizing Fluorescence Polarization. Biochim Biophys Acta. 1981 Jan 29;652(1):55-63. PubMed PMID: 7213736.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Competitive binding studies of compounds that interact with DNA utilizing fluorescence polarization. AU - Richardson,C L, AU - Schulman,G E, PY - 1981/1/29/pubmed PY - 1981/1/29/medline PY - 1981/1/29/entrez SP - 55 EP - 63 JF - Biochimica et biophysica acta JO - Biochim. Biophys. Acta VL - 652 IS - 1 N2 - The increase in the fluorescence polarization of acridine orange upon binding to DNA molecules is used as the basis of a competitive method to study the interaction of a variety of fluorescent and non-fluorescent compounds with DNA. Test compounds that interact with DNA inhibit both the binding of acridine orange to DNA and the accompanying increase in fluorescence polarization. Actinomycin D exhibits a dose-dependent inhibition of acridine orange-DNA binding with Micrococcus luteus DNA, calf thymus DNA, and poly(dG-dC); no detectable inhibition of acridine orange intercalation into poly(dA-dT) is observed. In contrast, proflavine shows similar acridine orange inhibition for poly(dA-dT), calf thymus DNA and M. luteus DNA. SN - 0006-3002 UR - https://www.unboundmedicine.com/medline/citation/7213736/Competitive_binding_studies_of_compounds_that_interact_with_DNA_utilizing_fluorescence_polarization_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0005-2787(81)90208-2 DB - PRIME DP - Unbound Medicine ER -