Tags

Type your tag names separated by a space and hit enter

[Purification and properties of glucose-6-phosphate and 6-phosphogluconate dehydrogenases from Pseudomonas oleovorans].
Biokhimiia. 1980 Aug; 45(8):1371-8.B

Abstract

The glucose-6-phosphate and 6-phosphogluconate dehydrogenases i. e. enzymes of dissimilatory hexulose phosphate cycle, were isolated from the cells of the facultative methylotrophic bacterium Pseudomonas oleovorans. The purification procedure included protein fractionation by ammonium sulfate, gel-filtration through Sephacryl S-200 and chromatography on DEAE Bio-Gel A and phosphocellulose, resulting in a 400-fold purification of the enzyme. During analytical disc-electrophoresis in polyacrylamide gel the glucose-6-phosphate dehydrogenase preparation produced a single band; 6-phosphogluconate dehydrogenase was found to contain small contaminations. The molecular weights of the dehydrogenases are 100000 and 110000, respectively. Both enzymes have two subunits. The Km values for glucose-6-phosphate dehydrogenase are 65 mkM for glucose-6-phosphate and 28 mkM for NADP; those for 6-phosphogluconate dehydrogenase are 152 mkM for 6-phosphogluconate and 55 mkM for NADP. Nucleotides are found to be the most active inhibitors of glucose-6-phosphate dehydrogenase. 6-Phosphogluconate dehydrogenase is inhibited by ribose-5-phosphate and fructose-1,6-diphosphate.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
English Abstract
Journal Article

Language

rus

PubMed ID

7236789

Citation

Sokolov, A P., et al. "[Purification and Properties of Glucose-6-phosphate and 6-phosphogluconate Dehydrogenases From Pseudomonas Oleovorans]." Biokhimiia (Moscow, Russia), vol. 45, no. 8, 1980, pp. 1371-8.
Sokolov AP, Luchin SV, Trotsenko IuA. [Purification and properties of glucose-6-phosphate and 6-phosphogluconate dehydrogenases from Pseudomonas oleovorans]. Biokhimiia. 1980;45(8):1371-8.
Sokolov, A. P., Luchin, S. V., & Trotsenko, I. u. A. (1980). [Purification and properties of glucose-6-phosphate and 6-phosphogluconate dehydrogenases from Pseudomonas oleovorans]. Biokhimiia (Moscow, Russia), 45(8), 1371-8.
Sokolov AP, Luchin SV, Trotsenko IuA. [Purification and Properties of Glucose-6-phosphate and 6-phosphogluconate Dehydrogenases From Pseudomonas Oleovorans]. Biokhimiia. 1980;45(8):1371-8. PubMed PMID: 7236789.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Purification and properties of glucose-6-phosphate and 6-phosphogluconate dehydrogenases from Pseudomonas oleovorans]. AU - Sokolov,A P, AU - Luchin,S V, AU - Trotsenko,Iu A, PY - 1980/8/1/pubmed PY - 1980/8/1/medline PY - 1980/8/1/entrez SP - 1371 EP - 8 JF - Biokhimiia (Moscow, Russia) JO - Biokhimiia VL - 45 IS - 8 N2 - The glucose-6-phosphate and 6-phosphogluconate dehydrogenases i. e. enzymes of dissimilatory hexulose phosphate cycle, were isolated from the cells of the facultative methylotrophic bacterium Pseudomonas oleovorans. The purification procedure included protein fractionation by ammonium sulfate, gel-filtration through Sephacryl S-200 and chromatography on DEAE Bio-Gel A and phosphocellulose, resulting in a 400-fold purification of the enzyme. During analytical disc-electrophoresis in polyacrylamide gel the glucose-6-phosphate dehydrogenase preparation produced a single band; 6-phosphogluconate dehydrogenase was found to contain small contaminations. The molecular weights of the dehydrogenases are 100000 and 110000, respectively. Both enzymes have two subunits. The Km values for glucose-6-phosphate dehydrogenase are 65 mkM for glucose-6-phosphate and 28 mkM for NADP; those for 6-phosphogluconate dehydrogenase are 152 mkM for 6-phosphogluconate and 55 mkM for NADP. Nucleotides are found to be the most active inhibitors of glucose-6-phosphate dehydrogenase. 6-Phosphogluconate dehydrogenase is inhibited by ribose-5-phosphate and fructose-1,6-diphosphate. SN - 0320-9725 UR - https://www.unboundmedicine.com/medline/citation/7236789/[Purification_and_properties_of_glucose_6_phosphate_and_6_phosphogluconate_dehydrogenases_from_Pseudomonas_oleovorans]_ DB - PRIME DP - Unbound Medicine ER -