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Changes in cation contents of stria vascularis with ouabain and potassium-free perfusion.
Hear Res. 1981 May; 4(2):149-60.HR

Abstract

Perfusion of the perilymphatic space of guinea pig cochleae with K-free medium leads to a gradual decline of the endocochlear potential (EP) over 30-50 min to a negative value (mean: -12 mV). The input resistance of scala media does not decrease during this time. The ATP and K content of the stria vascularis are reduced by similar amounts (26 and 34%, respectively) during this period. Perfusion of 1 mM ouabain produces a different pattern of response: strial ATP remains normal while strial K content is strongly reduced (by 77%). Strial Na rises in a complementary way to the K loss. These results demonstrate that a reduction of the K concentration of the perilymph leads to an inhibition of the generator of the positive component of the EP rather than to a general increase of cochlear duct membrane conductance. In addition, they suggest, in concert with other considerations (such as the slower rate of decline of the EP during K-free vascular perfusion (Wada, J., Kambayashi, J., Marcus, D.C. and Thalmann, R (1979): Arch. Otorhinolaryngol. 225, 79-81)), that the mode of action may be different from that of ouabain. In spite of the lack of teleological support, we offer the hypothesis that the strial generator of the EP may primarily utilize K from perilymph and that vascular K may not have access to the generator.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

7240022

Citation

Marcus, D C., et al. "Changes in Cation Contents of Stria Vascularis With Ouabain and Potassium-free Perfusion." Hearing Research, vol. 4, no. 2, 1981, pp. 149-60.
Marcus DC, Marcus NY, Thalmann R. Changes in cation contents of stria vascularis with ouabain and potassium-free perfusion. Hear Res. 1981;4(2):149-60.
Marcus, D. C., Marcus, N. Y., & Thalmann, R. (1981). Changes in cation contents of stria vascularis with ouabain and potassium-free perfusion. Hearing Research, 4(2), 149-60.
Marcus DC, Marcus NY, Thalmann R. Changes in Cation Contents of Stria Vascularis With Ouabain and Potassium-free Perfusion. Hear Res. 1981;4(2):149-60. PubMed PMID: 7240022.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Changes in cation contents of stria vascularis with ouabain and potassium-free perfusion. AU - Marcus,D C, AU - Marcus,N Y, AU - Thalmann,R, PY - 1981/5/1/pubmed PY - 1981/5/1/medline PY - 1981/5/1/entrez SP - 149 EP - 60 JF - Hearing research JO - Hear Res VL - 4 IS - 2 N2 - Perfusion of the perilymphatic space of guinea pig cochleae with K-free medium leads to a gradual decline of the endocochlear potential (EP) over 30-50 min to a negative value (mean: -12 mV). The input resistance of scala media does not decrease during this time. The ATP and K content of the stria vascularis are reduced by similar amounts (26 and 34%, respectively) during this period. Perfusion of 1 mM ouabain produces a different pattern of response: strial ATP remains normal while strial K content is strongly reduced (by 77%). Strial Na rises in a complementary way to the K loss. These results demonstrate that a reduction of the K concentration of the perilymph leads to an inhibition of the generator of the positive component of the EP rather than to a general increase of cochlear duct membrane conductance. In addition, they suggest, in concert with other considerations (such as the slower rate of decline of the EP during K-free vascular perfusion (Wada, J., Kambayashi, J., Marcus, D.C. and Thalmann, R (1979): Arch. Otorhinolaryngol. 225, 79-81)), that the mode of action may be different from that of ouabain. In spite of the lack of teleological support, we offer the hypothesis that the strial generator of the EP may primarily utilize K from perilymph and that vascular K may not have access to the generator. SN - 0378-5955 UR - https://www.unboundmedicine.com/medline/citation/7240022/Changes_in_cation_contents_of_stria_vascularis_with_ouabain_and_potassium_free_perfusion_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0378-5955(81)90002-2 DB - PRIME DP - Unbound Medicine ER -