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Reflectance spectrophotometric monitoring of the isolated perfused heart as a method of measuring the oxidation-reduction state of cytochromes and oxygenation of myoglobin.
Cardiovasc Res 1981; 15(2):86-91CR

Abstract

Reflectance spectrophotometry was assessed as a means of detecting metabolic oxidation-reduction changes in cytochromes and the oxygenation level of myoglobin in isolated perfused rat hearts. The movement artefact due to the heartbeat was largely eliminated by appropriate design of the optical geometry. A considerable myocardial oxygen gradient was detected using myoglobin deoxygenation as an indicator. The oxygenation level of myoglobin oscillated in pace with the contraction/relaxation cycle of the heart, deoxygenation occurring during the systole. The inconsistency in the previous reports on the myoglobin oxygenation and the problems of spectrophotometry of the beating heart are discussed.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

7260981

Citation

Hassinen, I E., et al. "Reflectance Spectrophotometric Monitoring of the Isolated Perfused Heart as a Method of Measuring the Oxidation-reduction State of Cytochromes and Oxygenation of Myoglobin." Cardiovascular Research, vol. 15, no. 2, 1981, pp. 86-91.
Hassinen IE, Hiltunen JK, Takala TE. Reflectance spectrophotometric monitoring of the isolated perfused heart as a method of measuring the oxidation-reduction state of cytochromes and oxygenation of myoglobin. Cardiovasc Res. 1981;15(2):86-91.
Hassinen, I. E., Hiltunen, J. K., & Takala, T. E. (1981). Reflectance spectrophotometric monitoring of the isolated perfused heart as a method of measuring the oxidation-reduction state of cytochromes and oxygenation of myoglobin. Cardiovascular Research, 15(2), pp. 86-91.
Hassinen IE, Hiltunen JK, Takala TE. Reflectance Spectrophotometric Monitoring of the Isolated Perfused Heart as a Method of Measuring the Oxidation-reduction State of Cytochromes and Oxygenation of Myoglobin. Cardiovasc Res. 1981;15(2):86-91. PubMed PMID: 7260981.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Reflectance spectrophotometric monitoring of the isolated perfused heart as a method of measuring the oxidation-reduction state of cytochromes and oxygenation of myoglobin. AU - Hassinen,I E, AU - Hiltunen,J K, AU - Takala,T E, PY - 1981/2/1/pubmed PY - 1981/2/1/medline PY - 1981/2/1/entrez SP - 86 EP - 91 JF - Cardiovascular research JO - Cardiovasc. Res. VL - 15 IS - 2 N2 - Reflectance spectrophotometry was assessed as a means of detecting metabolic oxidation-reduction changes in cytochromes and the oxygenation level of myoglobin in isolated perfused rat hearts. The movement artefact due to the heartbeat was largely eliminated by appropriate design of the optical geometry. A considerable myocardial oxygen gradient was detected using myoglobin deoxygenation as an indicator. The oxygenation level of myoglobin oscillated in pace with the contraction/relaxation cycle of the heart, deoxygenation occurring during the systole. The inconsistency in the previous reports on the myoglobin oxygenation and the problems of spectrophotometry of the beating heart are discussed. SN - 0008-6363 UR - https://www.unboundmedicine.com/medline/citation/7260981/Reflectance_spectrophotometric_monitoring_of_the_isolated_perfused_heart_as_a_method_of_measuring_the_oxidation_reduction_state_of_cytochromes_and_oxygenation_of_myoglobin_ L2 - https://academic.oup.com/cardiovascres/article-lookup/doi/10.1093/cvr/15.2.86 DB - PRIME DP - Unbound Medicine ER -