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Aconitase activity, citrate oxidation, and zinc inhibition in rat ventral prostate.
Enzyme. 1981; 26(6):281-7.E

Abstract

Aconitase activity and citrate oxidation were determined in mitochondrial preparations from rat ventral prostate. Aconitase activity in prostate mitochondria was approximately 10% of the activity observed in kidney preparations. The prostate aconitase activity was completely inhibited by the addition of 1.0 mmol/l zinc and kidney aconitase was inhibited by approximately 50%. The presence of 1.0 mmol/l zinc also markedly inhibited the formation of isocitrate from cis-aconitate. The reduction of NADP by citrate was markedly inhibited (approx. 95%) by 1.0 mmol/l zinc as compared with a 70 and 40% inhibition of aconitate--and isocitrate--stimulated reduction, respectively. These results indicate that zinc might be a strong inhibitor of aconitase activity with an inhibitory effect on isocitrate dehydrogenase also. The effect of 1.0 mmol/l zinc on citrate oxidation was studied by determining 14CO2 production from 6-14C-citrate. 14CO2 was significantly inhibited by zinc. These results further corroborate the conclusion that citrate accumulation in prostate might be the result of limited citrate oxidation which results from a combination of low aconitase activity and possibly zinc inhibition.

Authors

Costello LC
No affiliation info available
Franklin RB
No affiliation info available

MeSH

Aconitate HydrataseAnimalsCitratesCitric AcidKidneyMaleMitochondriaOxidation-ReductionProstateRatsZinc

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

7308179

Citation

Costello, L C., and R B. Franklin. "Aconitase Activity, Citrate Oxidation, and Zinc Inhibition in Rat Ventral Prostate." Enzyme, vol. 26, no. 6, 1981, pp. 281-7.
Costello LC, Franklin RB. Aconitase activity, citrate oxidation, and zinc inhibition in rat ventral prostate. Enzyme. 1981;26(6):281-7.
Costello, L. C., & Franklin, R. B. (1981). Aconitase activity, citrate oxidation, and zinc inhibition in rat ventral prostate. Enzyme, 26(6), 281-7.
Costello LC, Franklin RB. Aconitase Activity, Citrate Oxidation, and Zinc Inhibition in Rat Ventral Prostate. Enzyme. 1981;26(6):281-7. PubMed PMID: 7308179.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Aconitase activity, citrate oxidation, and zinc inhibition in rat ventral prostate. AU - Costello,L C, AU - Franklin,R B, PY - 1981/1/1/pubmed PY - 1981/1/1/medline PY - 1981/1/1/entrez SP - 281 EP - 7 JF - Enzyme JO - Enzyme VL - 26 IS - 6 N2 - Aconitase activity and citrate oxidation were determined in mitochondrial preparations from rat ventral prostate. Aconitase activity in prostate mitochondria was approximately 10% of the activity observed in kidney preparations. The prostate aconitase activity was completely inhibited by the addition of 1.0 mmol/l zinc and kidney aconitase was inhibited by approximately 50%. The presence of 1.0 mmol/l zinc also markedly inhibited the formation of isocitrate from cis-aconitate. The reduction of NADP by citrate was markedly inhibited (approx. 95%) by 1.0 mmol/l zinc as compared with a 70 and 40% inhibition of aconitate--and isocitrate--stimulated reduction, respectively. These results indicate that zinc might be a strong inhibitor of aconitase activity with an inhibitory effect on isocitrate dehydrogenase also. The effect of 1.0 mmol/l zinc on citrate oxidation was studied by determining 14CO2 production from 6-14C-citrate. 14CO2 was significantly inhibited by zinc. These results further corroborate the conclusion that citrate accumulation in prostate might be the result of limited citrate oxidation which results from a combination of low aconitase activity and possibly zinc inhibition. SN - 0013-9432 UR - https://www.unboundmedicine.com/medline/citation/7308179/Aconitase_activity_citrate_oxidation_and_zinc_inhibition_in_rat_ventral_prostate_ DB - PRIME DP - Unbound Medicine ER -