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A multi-wavelength spectrophotometric method for the simultaneous determination of five haemoglobin derivatives.
J Clin Chem Clin Biochem. 1981 Jul; 19(7):457-63.JC

Abstract

A method is described by which the concentration of deoxyhaemoglobin, oxyhaemoglobin, carboxyhaemoglobin, haemoglobin and sulfhaemoglobin in a human blood sample is determined by passing the haemolysate without air contact through a coarse filter and subsequently measuring the absorbance at lambda = 500, 569, 577, 620 and 760 nm. The ensuing set of equations is solved by matrix calculation with the aid of a simple computer program. The method has been tested by comparing it with conventional methods for the determination of the various haemoglobin derivatives separately.

Authors

Zwart A
No affiliation info available
Buursma A
No affiliation info available
van Kampen EJ
No affiliation info available
Oeseburg B
No affiliation info available
van der Ploeg PH
No affiliation info available
Zijlstra WG
No affiliation info available

MeSH

CarboxyhemoglobinComputersHemoglobinsHumansMathematicsOxyhemoglobinsSpectrophotometrySulfhemoglobin

Pub Type(s)

Journal Article

Language

eng

PubMed ID

7328392

Citation

Zwart, A, et al. "A Multi-wavelength Spectrophotometric Method for the Simultaneous Determination of Five Haemoglobin Derivatives." Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Fur Klinische Chemie Und Klinische Biochemie, vol. 19, no. 7, 1981, pp. 457-63.
Zwart A, Buursma A, van Kampen EJ, et al. A multi-wavelength spectrophotometric method for the simultaneous determination of five haemoglobin derivatives. J Clin Chem Clin Biochem. 1981;19(7):457-63.
Zwart, A., Buursma, A., van Kampen, E. J., Oeseburg, B., van der Ploeg, P. H., & Zijlstra, W. G. (1981). A multi-wavelength spectrophotometric method for the simultaneous determination of five haemoglobin derivatives. Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Fur Klinische Chemie Und Klinische Biochemie, 19(7), 457-63.
Zwart A, et al. A Multi-wavelength Spectrophotometric Method for the Simultaneous Determination of Five Haemoglobin Derivatives. J Clin Chem Clin Biochem. 1981;19(7):457-63. PubMed PMID: 7328392.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A multi-wavelength spectrophotometric method for the simultaneous determination of five haemoglobin derivatives. AU - Zwart,A, AU - Buursma,A, AU - van Kampen,E J, AU - Oeseburg,B, AU - van der Ploeg,P H, AU - Zijlstra,W G, PY - 1981/7/1/pubmed PY - 1981/7/1/medline PY - 1981/7/1/entrez SP - 457 EP - 63 JF - Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie JO - J Clin Chem Clin Biochem VL - 19 IS - 7 N2 - A method is described by which the concentration of deoxyhaemoglobin, oxyhaemoglobin, carboxyhaemoglobin, haemoglobin and sulfhaemoglobin in a human blood sample is determined by passing the haemolysate without air contact through a coarse filter and subsequently measuring the absorbance at lambda = 500, 569, 577, 620 and 760 nm. The ensuing set of equations is solved by matrix calculation with the aid of a simple computer program. The method has been tested by comparing it with conventional methods for the determination of the various haemoglobin derivatives separately. SN - 0340-076X UR - https://www.unboundmedicine.com/medline/citation/7328392/A_multi_wavelength_spectrophotometric_method_for_the_simultaneous_determination_of_five_haemoglobin_derivatives_ L2 - https://www.lens.org/lens/search/patent/list?q=citation_id:7328392 DB - PRIME DP - Unbound Medicine ER -