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Effects of ethanol and related drugs on the physical and functional properties of brain membranes.
Curr Alcohol. 1981; 8:461-8.CA

Abstract

The fluorescent probe 1,6-diphenyl-1,3,5-hexatriene (DPH) was incorporated into myelin and synaptic plasma membrane fractions prepared from mouse brain. The absorption-corrected fluorescence and polarization of the fluorescent probe a molecule and the membrane absorbance were measured simultaneously, using a computer-centered spectrofluorimeter. In vitro exposure of synaptic membranes to ethanol (25-100 mM) decreased all three parameters in a dose-dependent manner. The order of sensitivity was: corrected fluorescence greater than or equal to polarization much greater than absorbance. Myelin was not affected by these concentrations of ethanol, indicating a selective effect on synaptic membranes. The polarization of fluorescence of DPH in synaptic membranes was also decreased by tert-butanol, pentobarbital, and cis- and trans-vaccinic acid. The results suggest that ethanol and related drugs perturb the hydrophobic interior of synaptic membranes. These compounds also increased the exposure of synaptic sulfhydryl groups and inhibited the depolarization- and ATP-dependent uptake of calcium by synaptosomal preparations. These results suggest that the perturbation altered the conformation of synaptic proteins and inhibited the transport of calcium by synaptosomal membranes.

Authors

No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

7343190

Citation

Harris, R A., and F Schroeder. "Effects of Ethanol and Related Drugs On the Physical and Functional Properties of Brain Membranes." Currents in Alcoholism, vol. 8, 1981, pp. 461-8.
Harris RA, Schroeder F. Effects of ethanol and related drugs on the physical and functional properties of brain membranes. Curr Alcohol. 1981;8:461-8.
Harris, R. A., & Schroeder, F. (1981). Effects of ethanol and related drugs on the physical and functional properties of brain membranes. Currents in Alcoholism, 8, 461-8.
Harris RA, Schroeder F. Effects of Ethanol and Related Drugs On the Physical and Functional Properties of Brain Membranes. Curr Alcohol. 1981;8:461-8. PubMed PMID: 7343190.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of ethanol and related drugs on the physical and functional properties of brain membranes. AU - Harris,R A, AU - Schroeder,F, PY - 1981/1/1/pubmed PY - 1981/1/1/medline PY - 1981/1/1/entrez SP - 461 EP - 8 JF - Currents in alcoholism JO - Curr Alcohol VL - 8 N2 - The fluorescent probe 1,6-diphenyl-1,3,5-hexatriene (DPH) was incorporated into myelin and synaptic plasma membrane fractions prepared from mouse brain. The absorption-corrected fluorescence and polarization of the fluorescent probe a molecule and the membrane absorbance were measured simultaneously, using a computer-centered spectrofluorimeter. In vitro exposure of synaptic membranes to ethanol (25-100 mM) decreased all three parameters in a dose-dependent manner. The order of sensitivity was: corrected fluorescence greater than or equal to polarization much greater than absorbance. Myelin was not affected by these concentrations of ethanol, indicating a selective effect on synaptic membranes. The polarization of fluorescence of DPH in synaptic membranes was also decreased by tert-butanol, pentobarbital, and cis- and trans-vaccinic acid. The results suggest that ethanol and related drugs perturb the hydrophobic interior of synaptic membranes. These compounds also increased the exposure of synaptic sulfhydryl groups and inhibited the depolarization- and ATP-dependent uptake of calcium by synaptosomal preparations. These results suggest that the perturbation altered the conformation of synaptic proteins and inhibited the transport of calcium by synaptosomal membranes. SN - 0161-8504 UR - https://www.unboundmedicine.com/medline/citation/7343190/Effects_of_ethanol_and_related_drugs_on_the_physical_and_functional_properties_of_brain_membranes_ DB - PRIME DP - Unbound Medicine ER -