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Blocking of histamine release from human basophils in vitro by the ATPase inhibitor, ethacrynic acid.
Clin Exp Immunol. 1977 Sep; 29(3):436-41.CE

Abstract

Ethacrynic acid, a known inhibitor of both Na+--K+ and Mg2+-activated ATPases, effectively inhibits histamine release from antigen-challenged human basophils in vitro. Ouabain, an inhibitor specific for Na+--K+-activated ATPases, shows no effect upon the quantity of histamine released from the antigen-challenged basophils. Ethacrynic acid also effectively inhibits Ca2+--ionophore A23187-induced release, implying it inhibits the Ca2+-dependent secretory stage of the histamine-release process. Inhibition of ATPases and histamine release by ethacrynic acid both require the presence of the olefinic bond in the ethacrynic-acid molecule. Possible utilization of analogues of ethacrynic acid as anti-allergic drugs and as a device to investigate the ATPase system of histamine-releasing cells is suggested.

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Authors

Magro AM
No affiliation info available

MeSH

Adenosine TriphosphatasesBasophilsCalcimycinCysteineDose-Response Relationship, ImmunologicEthacrynic AcidHistamine ReleaseHumansImmunoglobulin EIn Vitro TechniquesOuabainSulfhydryl Compounds

Pub Type(s)

Comparative Study
Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

73437

Citation

Magro, A M.. "Blocking of Histamine Release From Human Basophils in Vitro By the ATPase Inhibitor, Ethacrynic Acid." Clinical and Experimental Immunology, vol. 29, no. 3, 1977, pp. 436-41.
Magro AM. Blocking of histamine release from human basophils in vitro by the ATPase inhibitor, ethacrynic acid. Clin Exp Immunol. 1977;29(3):436-41.
Magro, A. M. (1977). Blocking of histamine release from human basophils in vitro by the ATPase inhibitor, ethacrynic acid. Clinical and Experimental Immunology, 29(3), 436-41.
Magro AM. Blocking of Histamine Release From Human Basophils in Vitro By the ATPase Inhibitor, Ethacrynic Acid. Clin Exp Immunol. 1977;29(3):436-41. PubMed PMID: 73437.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Blocking of histamine release from human basophils in vitro by the ATPase inhibitor, ethacrynic acid. A1 - Magro,A M, PY - 1977/9/1/pubmed PY - 1977/9/1/medline PY - 1977/9/1/entrez SP - 436 EP - 41 JF - Clinical and experimental immunology JO - Clin Exp Immunol VL - 29 IS - 3 N2 - Ethacrynic acid, a known inhibitor of both Na+--K+ and Mg2+-activated ATPases, effectively inhibits histamine release from antigen-challenged human basophils in vitro. Ouabain, an inhibitor specific for Na+--K+-activated ATPases, shows no effect upon the quantity of histamine released from the antigen-challenged basophils. Ethacrynic acid also effectively inhibits Ca2+--ionophore A23187-induced release, implying it inhibits the Ca2+-dependent secretory stage of the histamine-release process. Inhibition of ATPases and histamine release by ethacrynic acid both require the presence of the olefinic bond in the ethacrynic-acid molecule. Possible utilization of analogues of ethacrynic acid as anti-allergic drugs and as a device to investigate the ATPase system of histamine-releasing cells is suggested. SN - 0009-9104 UR - https://www.unboundmedicine.com/medline/citation/73437/Blocking_of_histamine_release_from_human_basophils_in_vitro_by_the_ATPase_inhibitor_ethacrynic_acid_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/73437/ DB - PRIME DP - Unbound Medicine ER -