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Deformability of oxygenated irreversibly sickled cells.
J Clin Invest. 1980 Jan; 65(1):189-96.JCI

Abstract

The deformability characteristics of isolated subpopulations of irreversibly sickled cells (ISC) have been studied in an ektacytometer. Analysis of laser diffraction patterns of well-oxygenated cells subjected to shear stress in solutions of varying osmolality has demonstrated a profound influence of mean corpuscular hemoglobin concentration and intracellular viscosity on the deformability of ISC. Virtually undeformable at 290 mosM, ISC became almost totally deformable at 130 mosM. In addition, when ISC membranes were loaded with normal hemoglobin at low concentration, they deformed easily in isotonic medium, as did resealed normal cell membranes. The restoration of deformability of ISC upon reduction of their hemoglobin concentration and internal viscosity to normal levels suggests that altered membrane properties are not the primary determinant of decreased deformability in these cells. Rather, cellular dehydration induced by previous sickling would appear to contribute in a major way to their abnormal rheological behavior.

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Authors

Clark MR
No affiliation info available
Mohandas N
No affiliation info available
Shohet SB
No affiliation info available

MeSH

Anemia, Sickle CellBlood ViscosityErythrocytes, AbnormalHemoglobinsHumansLasersMembrane FluidityOsmotic PressureOxygen

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

7350198

Citation

Clark, M R., et al. "Deformability of Oxygenated Irreversibly Sickled Cells." The Journal of Clinical Investigation, vol. 65, no. 1, 1980, pp. 189-96.
Clark MR, Mohandas N, Shohet SB. Deformability of oxygenated irreversibly sickled cells. J Clin Invest. 1980;65(1):189-96.
Clark, M. R., Mohandas, N., & Shohet, S. B. (1980). Deformability of oxygenated irreversibly sickled cells. The Journal of Clinical Investigation, 65(1), 189-96.
Clark MR, Mohandas N, Shohet SB. Deformability of Oxygenated Irreversibly Sickled Cells. J Clin Invest. 1980;65(1):189-96. PubMed PMID: 7350198.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Deformability of oxygenated irreversibly sickled cells. AU - Clark,M R, AU - Mohandas,N, AU - Shohet,S B, PY - 1980/1/1/pubmed PY - 1980/1/1/medline PY - 1980/1/1/entrez SP - 189 EP - 96 JF - The Journal of clinical investigation JO - J Clin Invest VL - 65 IS - 1 N2 - The deformability characteristics of isolated subpopulations of irreversibly sickled cells (ISC) have been studied in an ektacytometer. Analysis of laser diffraction patterns of well-oxygenated cells subjected to shear stress in solutions of varying osmolality has demonstrated a profound influence of mean corpuscular hemoglobin concentration and intracellular viscosity on the deformability of ISC. Virtually undeformable at 290 mosM, ISC became almost totally deformable at 130 mosM. In addition, when ISC membranes were loaded with normal hemoglobin at low concentration, they deformed easily in isotonic medium, as did resealed normal cell membranes. The restoration of deformability of ISC upon reduction of their hemoglobin concentration and internal viscosity to normal levels suggests that altered membrane properties are not the primary determinant of decreased deformability in these cells. Rather, cellular dehydration induced by previous sickling would appear to contribute in a major way to their abnormal rheological behavior. SN - 0021-9738 UR - https://www.unboundmedicine.com/medline/citation/7350198/Deformability_of_oxygenated_irreversibly_sickled_cells_ L2 - https://doi.org/10.1172/JCI109650 DB - PRIME DP - Unbound Medicine ER -