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Trans-complementable copy-number mutants of plasmid ColE1.
Nature. 1980 Jan 10; 283(5743):216-8.Nat

Abstract

Plasmid ColE1, like many other small non-conjugative plasmids, is present in multiple copies (about 15 per chromsome equivalent) in Escherichia coli cells. Because of their high copy number, the replication of such plasmids has been described as 'relaxed', even though there is good evidence that it is strictly controlled: ColE1 derivatives have characteristic but different copy numbers and ColE1 copy-number mutants have been characterised. No plasmid-specified protein is essential for the replication of ColE1 and related plasmids, as extensive replication can occur in chloramphenicol-treated cells, in plasmid-free chloramphenicol-treated cells transfected with a hybrid ColE1/phage replicon and in vitro in extracts derived from plasmid-free cells. Nevertheless, it is possible that a plasmid-specified protein is involved in ColE1 replication control in viable cells. Here we show that deletion of a given non-essential region from ColE1-like plasmids results in a raised copy number. Such plasmids are stably maintained and have their copy number returned to normal when a complementing plasmid is present in the same cell, indicating that a plasmid-specified diffusible gene product regulates the plasmid content of ColE1-containing cells. Deletion of the equivalent region from the cloning vector pBR322 gives a derivative which has a raised copy number and which has also lost its origin for conjugal transfer; unlike pBR322, it cannot be mobilised.

Authors

No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

7350544

Citation

Twigg, A J., and D Sherratt. "Trans-complementable Copy-number Mutants of Plasmid ColE1." Nature, vol. 283, no. 5743, 1980, pp. 216-8.
Twigg AJ, Sherratt D. Trans-complementable copy-number mutants of plasmid ColE1. Nature. 1980;283(5743):216-8.
Twigg, A. J., & Sherratt, D. (1980). Trans-complementable copy-number mutants of plasmid ColE1. Nature, 283(5743), 216-8.
Twigg AJ, Sherratt D. Trans-complementable Copy-number Mutants of Plasmid ColE1. Nature. 1980 Jan 10;283(5743):216-8. PubMed PMID: 7350544.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Trans-complementable copy-number mutants of plasmid ColE1. AU - Twigg,A J, AU - Sherratt,D, PY - 1980/1/10/pubmed PY - 1980/1/10/medline PY - 1980/1/10/entrez SP - 216 EP - 8 JF - Nature JO - Nature VL - 283 IS - 5743 N2 - Plasmid ColE1, like many other small non-conjugative plasmids, is present in multiple copies (about 15 per chromsome equivalent) in Escherichia coli cells. Because of their high copy number, the replication of such plasmids has been described as 'relaxed', even though there is good evidence that it is strictly controlled: ColE1 derivatives have characteristic but different copy numbers and ColE1 copy-number mutants have been characterised. No plasmid-specified protein is essential for the replication of ColE1 and related plasmids, as extensive replication can occur in chloramphenicol-treated cells, in plasmid-free chloramphenicol-treated cells transfected with a hybrid ColE1/phage replicon and in vitro in extracts derived from plasmid-free cells. Nevertheless, it is possible that a plasmid-specified protein is involved in ColE1 replication control in viable cells. Here we show that deletion of a given non-essential region from ColE1-like plasmids results in a raised copy number. Such plasmids are stably maintained and have their copy number returned to normal when a complementing plasmid is present in the same cell, indicating that a plasmid-specified diffusible gene product regulates the plasmid content of ColE1-containing cells. Deletion of the equivalent region from the cloning vector pBR322 gives a derivative which has a raised copy number and which has also lost its origin for conjugal transfer; unlike pBR322, it cannot be mobilised. SN - 0028-0836 UR - https://www.unboundmedicine.com/medline/citation/7350544/Trans_complementable_copy_number_mutants_of_plasmid_ColE1_ L2 - https://www.lens.org/lens/search?q=citation_id:7350544 DB - PRIME DP - Unbound Medicine ER -