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Purification and properties of glucose-6-phosphate dehydrogenase (NADP+/NAD+) and 6-phosphogluconate dehydrogenase (NADP+/NAD+) from methanol-grown Pseudomonas C.
Biochim Biophys Acta. 1980 Jan 11; 611(1):1-10.BB

Abstract

Glucose-6-phosphate dehydrogenase (D-glucose-6-phosphate:NADPH+ 1-oxidoreductase, EC 1.1.1.49) and 6-phosphogluconate dehydrogenase (6-phospho-D-gluconate:NADP+ 2-oxidoreductase, EC 1.1.1943) have been purified from methanol-grown Pseudomonas C. Glucose-6-phosphate dehydrogenase exhibits activity with either NADP+ or NAD+ as coenzymes, V NADP+ = 0.96 V NAD+.Km values of 22, 290, and 250 microns are obtained for NADP+, NAD+ and glucose 6-phosphate (NADP+ as the coenzyme), respectively. ATP inhibits Glc-6P dehydrogenase activity with NAD+ as coenzyme and to a less extent the activity with DANP+. In the presence of MgCl2, ATP inhibition of Blc-6P dehydrogeanse activity is abolished. 6-Phosphogluconate dehydrogenase has a dual specificity for both NADP+ or NAD+ as coenzymes, V NADP+ = 1.66 V NAD+.Km values of 20, 500 and 100 microns are obtained for NADP+, NAD+ and 6-phosphogluconate (NADP+ as the coenzyme), respectively. With NAD+ as the coenzyme ATP inhibits 6-phosphogluconate dehydrogeanse activity, while with NADP+ as the coenzyme, activity was less affected. The possible role of these enzymes in the metabolism of one-carbon (C1)-compounds in Pseudomonas C is discussed and compared with other methylotrophic microorganisms.

Authors

No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

7350909

Citation

Ben-Bassat, A, and I Goldberg. "Purification and Properties of Glucose-6-phosphate Dehydrogenase (NADP+/NAD+) and 6-phosphogluconate Dehydrogenase (NADP+/NAD+) From Methanol-grown Pseudomonas C." Biochimica Et Biophysica Acta, vol. 611, no. 1, 1980, pp. 1-10.
Ben-Bassat A, Goldberg I. Purification and properties of glucose-6-phosphate dehydrogenase (NADP+/NAD+) and 6-phosphogluconate dehydrogenase (NADP+/NAD+) from methanol-grown Pseudomonas C. Biochim Biophys Acta. 1980;611(1):1-10.
Ben-Bassat, A., & Goldberg, I. (1980). Purification and properties of glucose-6-phosphate dehydrogenase (NADP+/NAD+) and 6-phosphogluconate dehydrogenase (NADP+/NAD+) from methanol-grown Pseudomonas C. Biochimica Et Biophysica Acta, 611(1), 1-10.
Ben-Bassat A, Goldberg I. Purification and Properties of Glucose-6-phosphate Dehydrogenase (NADP+/NAD+) and 6-phosphogluconate Dehydrogenase (NADP+/NAD+) From Methanol-grown Pseudomonas C. Biochim Biophys Acta. 1980 Jan 11;611(1):1-10. PubMed PMID: 7350909.
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TY - JOUR T1 - Purification and properties of glucose-6-phosphate dehydrogenase (NADP+/NAD+) and 6-phosphogluconate dehydrogenase (NADP+/NAD+) from methanol-grown Pseudomonas C. AU - Ben-Bassat,A, AU - Goldberg,I, PY - 1980/1/11/pubmed PY - 1980/1/11/medline PY - 1980/1/11/entrez SP - 1 EP - 10 JF - Biochimica et biophysica acta JO - Biochim. Biophys. Acta VL - 611 IS - 1 N2 - Glucose-6-phosphate dehydrogenase (D-glucose-6-phosphate:NADPH+ 1-oxidoreductase, EC 1.1.1.49) and 6-phosphogluconate dehydrogenase (6-phospho-D-gluconate:NADP+ 2-oxidoreductase, EC 1.1.1943) have been purified from methanol-grown Pseudomonas C. Glucose-6-phosphate dehydrogenase exhibits activity with either NADP+ or NAD+ as coenzymes, V NADP+ = 0.96 V NAD+.Km values of 22, 290, and 250 microns are obtained for NADP+, NAD+ and glucose 6-phosphate (NADP+ as the coenzyme), respectively. ATP inhibits Glc-6P dehydrogenase activity with NAD+ as coenzyme and to a less extent the activity with DANP+. In the presence of MgCl2, ATP inhibition of Blc-6P dehydrogeanse activity is abolished. 6-Phosphogluconate dehydrogenase has a dual specificity for both NADP+ or NAD+ as coenzymes, V NADP+ = 1.66 V NAD+.Km values of 20, 500 and 100 microns are obtained for NADP+, NAD+ and 6-phosphogluconate (NADP+ as the coenzyme), respectively. With NAD+ as the coenzyme ATP inhibits 6-phosphogluconate dehydrogeanse activity, while with NADP+ as the coenzyme, activity was less affected. The possible role of these enzymes in the metabolism of one-carbon (C1)-compounds in Pseudomonas C is discussed and compared with other methylotrophic microorganisms. SN - 0006-3002 UR - https://www.unboundmedicine.com/medline/citation/7350909/Purification_and_properties_of_glucose_6_phosphate_dehydrogenase__NADP+/NAD+__and_6_phosphogluconate_dehydrogenase__NADP+/NAD+__from_methanol_grown_Pseudomonas_C_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0005-2744(80)90036-4 DB - PRIME DP - Unbound Medicine ER -