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Effects of cannabinoids on catecholamine uptake and release in hypothalamic and striatal synaptosomes.
J Pharmacol Exp Ther. 1980 Jul; 214(1):63-7.JP

Abstract

In vitro effects of delta 9-tetrahydrocannabinol (delta 9-THC), delta 8-tetrahydrocannabinol (delta 8-THC), cannabinol (CBN) and cannabidiol (CBD) have been studied on the uptake and release of 3H-labeled dopamine (DA) and norepinephrine (NE) in the synaptosomal preparation of rat brain corpus striatum and hypothalamus. The uptake of both DA and NE in these two regions was stimulated at low concentrations of delta 9-THC (1 x 10(-7) and 2 x 10(-7) M) and delta 8-THC (5 x 10(-9) and 1 x 10(-8) M), whereas at higher concentrations (1 x 10(-5) and 1 x 10(-4) M) both delta 9-THC and its delta 8-isomer inhibited the uptake of DA and NE. Similarly, the release of DA and NE from the preloaded synaptosomes of these two brain regions were inhibited at low concentrations of delta 9-THC (1 x 10(-7) M) and delta 8-THC (1 x 10(-8) M) and stimulated at high concentrations of both isomers (1 x 10(-5) and 1 x 10(-4) M). High concentrations of both CBN and CBD were needed to produce only an inhibitory effect on the uptake and the stimulation on the release of DA and NE in synaptosomes of the two brain regions. No significant effect was found at lower concentrations of CBN and CBD. These results demonstrate that 1) delta 9- and delta 8-THC, but not CBN and CBD, produce a biphasic effect on the uptake and release of DA and NE in the corpus striatum and hypothalamic regions of brain and 2) delta 8-THC is more potent than delta 9-THC on both uptake and release of DA and NE in these two brain regions.

Authors

No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

7391971

Citation

Poddar, M K., and W L. Dewey. "Effects of Cannabinoids On Catecholamine Uptake and Release in Hypothalamic and Striatal Synaptosomes." The Journal of Pharmacology and Experimental Therapeutics, vol. 214, no. 1, 1980, pp. 63-7.
Poddar MK, Dewey WL. Effects of cannabinoids on catecholamine uptake and release in hypothalamic and striatal synaptosomes. J Pharmacol Exp Ther. 1980;214(1):63-7.
Poddar, M. K., & Dewey, W. L. (1980). Effects of cannabinoids on catecholamine uptake and release in hypothalamic and striatal synaptosomes. The Journal of Pharmacology and Experimental Therapeutics, 214(1), 63-7.
Poddar MK, Dewey WL. Effects of Cannabinoids On Catecholamine Uptake and Release in Hypothalamic and Striatal Synaptosomes. J Pharmacol Exp Ther. 1980;214(1):63-7. PubMed PMID: 7391971.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of cannabinoids on catecholamine uptake and release in hypothalamic and striatal synaptosomes. AU - Poddar,M K, AU - Dewey,W L, PY - 1980/7/1/pubmed PY - 1980/7/1/medline PY - 1980/7/1/entrez SP - 63 EP - 7 JF - The Journal of pharmacology and experimental therapeutics JO - J Pharmacol Exp Ther VL - 214 IS - 1 N2 - In vitro effects of delta 9-tetrahydrocannabinol (delta 9-THC), delta 8-tetrahydrocannabinol (delta 8-THC), cannabinol (CBN) and cannabidiol (CBD) have been studied on the uptake and release of 3H-labeled dopamine (DA) and norepinephrine (NE) in the synaptosomal preparation of rat brain corpus striatum and hypothalamus. The uptake of both DA and NE in these two regions was stimulated at low concentrations of delta 9-THC (1 x 10(-7) and 2 x 10(-7) M) and delta 8-THC (5 x 10(-9) and 1 x 10(-8) M), whereas at higher concentrations (1 x 10(-5) and 1 x 10(-4) M) both delta 9-THC and its delta 8-isomer inhibited the uptake of DA and NE. Similarly, the release of DA and NE from the preloaded synaptosomes of these two brain regions were inhibited at low concentrations of delta 9-THC (1 x 10(-7) M) and delta 8-THC (1 x 10(-8) M) and stimulated at high concentrations of both isomers (1 x 10(-5) and 1 x 10(-4) M). High concentrations of both CBN and CBD were needed to produce only an inhibitory effect on the uptake and the stimulation on the release of DA and NE in synaptosomes of the two brain regions. No significant effect was found at lower concentrations of CBN and CBD. These results demonstrate that 1) delta 9- and delta 8-THC, but not CBN and CBD, produce a biphasic effect on the uptake and release of DA and NE in the corpus striatum and hypothalamic regions of brain and 2) delta 8-THC is more potent than delta 9-THC on both uptake and release of DA and NE in these two brain regions. SN - 0022-3565 UR - https://www.unboundmedicine.com/medline/citation/7391971/Effects_of_cannabinoids_on_catecholamine_uptake_and_release_in_hypothalamic_and_striatal_synaptosomes_ L2 - https://jpet.aspetjournals.org/cgi/pmidlookup?view=long&pmid=7391971 DB - PRIME DP - Unbound Medicine ER -