Tags

Type your tag names separated by a space and hit enter

Glyoxylate cycle in Mucor racemosus.
J Bacteriol. 1980 Jul; 143(1):416-21.JB

Abstract

The dimorphic phycomycete Mucor racemosus was grown in media containing acetate, glutamate, and peptone as carbon sources. The component enzymes of the glyoxylate bypass, isocitrate lyase and malate synthase, were present under these conditions throughout the growth cycles. Highest specific activities for each enzyme were found in media with acetate as the carbon source. In an enriched peptone medium containing glucose, neither activity was detected until glucose was exhausted from the medium. Treatment of acetate-grown cells with glucose resulted in a rapid decline in the specific activities of both enzymes. The importance of this cycle in acetate-grown cells was indicated by the ability of itaconic acid (100 mM) to inhibit the growth of M. racemosus in acetate but not glutamate media. Itaconate was also shown to be a potent inhibitor of isocitrate lyase activity in vitro.

Links

PMC Free PDF
PMC Free Full Text
Publisher Full Text
journals.asm.org
jb.asm.org

Authors

O'Connell BT
No affiliation info available
Paznokas JL
No affiliation info available

MeSH

AcetatesAmino AcidsCulture MediaGlutamatesIsocitrate LyaseKetoglutarate Dehydrogenase ComplexMalate SynthaseMucorOxo-Acid-LyasesSuccinates

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

7400097

Citation

O'Connell, B T., and J L. Paznokas. "Glyoxylate Cycle in Mucor Racemosus." Journal of Bacteriology, vol. 143, no. 1, 1980, pp. 416-21.
O'Connell BT, Paznokas JL. Glyoxylate cycle in Mucor racemosus. J Bacteriol. 1980;143(1):416-21.
O'Connell, B. T., & Paznokas, J. L. (1980). Glyoxylate cycle in Mucor racemosus. Journal of Bacteriology, 143(1), 416-21.
O'Connell BT, Paznokas JL. Glyoxylate Cycle in Mucor Racemosus. J Bacteriol. 1980;143(1):416-21. PubMed PMID: 7400097.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Glyoxylate cycle in Mucor racemosus. AU - O'Connell,B T, AU - Paznokas,J L, PY - 1980/7/1/pubmed PY - 1980/7/1/medline PY - 1980/7/1/entrez SP - 416 EP - 21 JF - Journal of bacteriology JO - J Bacteriol VL - 143 IS - 1 N2 - The dimorphic phycomycete Mucor racemosus was grown in media containing acetate, glutamate, and peptone as carbon sources. The component enzymes of the glyoxylate bypass, isocitrate lyase and malate synthase, were present under these conditions throughout the growth cycles. Highest specific activities for each enzyme were found in media with acetate as the carbon source. In an enriched peptone medium containing glucose, neither activity was detected until glucose was exhausted from the medium. Treatment of acetate-grown cells with glucose resulted in a rapid decline in the specific activities of both enzymes. The importance of this cycle in acetate-grown cells was indicated by the ability of itaconic acid (100 mM) to inhibit the growth of M. racemosus in acetate but not glutamate media. Itaconate was also shown to be a potent inhibitor of isocitrate lyase activity in vitro. SN - 0021-9193 UR - https://www.unboundmedicine.com/medline/citation/7400097/Glyoxylate_cycle_in_Mucor_racemosus_ L2 - https://journals.asm.org/doi/10.1128/jb.143.1.416-421.1980?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -