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Role and control of isocitrate lyase in Candida lipolytica.
J Bacteriol. 1980 Nov; 144(2):692-7.JB

Abstract

Mutants of Candida lipolytica that were unable to grow on acetate but able to utilize succinate or glycerol as a sole carbon source were isolated. Amongst the mutants isolated, one strain (Icl-) was specifically deficient in isocitrate lyase activity, whereas another strain (Acos-) was deficient in acetyl coenzyme A synthetase activity. Since the Icl- mutant could not grow either on n-alkane or its derivatives, such as fatty acid and long-chain dicarboxylic acid, any anaplerotic route other than the glyoxylate pathway was inconceivable as far as growth on these carbon sources was concerned. Acetyl coenzyme A is most likely a metabolic inducer of isocitrate lyase and malate synthase, because the Acos- mutant was characterized by the least susceptibility to induction of these enzymes by acetate. The structural gene for isocitrate lyase was most probably impaired in the Icl- mutant, since revertants (Icl-) produced thermolabile isocitrate lyase. The production of isocitrate from n-alkane by the revertants was enhanced in comparison with the parental strain.

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Authors

Matsuoka M
No affiliation info available
Ueda Y
No affiliation info available
Aiba S
No affiliation info available

MeSH

Acetyl Coenzyme AAlkanesCandidaCitratesEnzyme InductionIsocitrate LyaseIsocitratesMalate SynthaseMutationOxo-Acid-Lyases

Pub Type(s)

Journal Article

Language

eng

PubMed ID

7430068

Citation

Matsuoka, M, et al. "Role and Control of Isocitrate Lyase in Candida Lipolytica." Journal of Bacteriology, vol. 144, no. 2, 1980, pp. 692-7.
Matsuoka M, Ueda Y, Aiba S. Role and control of isocitrate lyase in Candida lipolytica. J Bacteriol. 1980;144(2):692-7.
Matsuoka, M., Ueda, Y., & Aiba, S. (1980). Role and control of isocitrate lyase in Candida lipolytica. Journal of Bacteriology, 144(2), 692-7.
Matsuoka M, Ueda Y, Aiba S. Role and Control of Isocitrate Lyase in Candida Lipolytica. J Bacteriol. 1980;144(2):692-7. PubMed PMID: 7430068.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Role and control of isocitrate lyase in Candida lipolytica. AU - Matsuoka,M, AU - Ueda,Y, AU - Aiba,S, PY - 1980/11/1/pubmed PY - 1980/11/1/medline PY - 1980/11/1/entrez SP - 692 EP - 7 JF - Journal of bacteriology JO - J Bacteriol VL - 144 IS - 2 N2 - Mutants of Candida lipolytica that were unable to grow on acetate but able to utilize succinate or glycerol as a sole carbon source were isolated. Amongst the mutants isolated, one strain (Icl-) was specifically deficient in isocitrate lyase activity, whereas another strain (Acos-) was deficient in acetyl coenzyme A synthetase activity. Since the Icl- mutant could not grow either on n-alkane or its derivatives, such as fatty acid and long-chain dicarboxylic acid, any anaplerotic route other than the glyoxylate pathway was inconceivable as far as growth on these carbon sources was concerned. Acetyl coenzyme A is most likely a metabolic inducer of isocitrate lyase and malate synthase, because the Acos- mutant was characterized by the least susceptibility to induction of these enzymes by acetate. The structural gene for isocitrate lyase was most probably impaired in the Icl- mutant, since revertants (Icl-) produced thermolabile isocitrate lyase. The production of isocitrate from n-alkane by the revertants was enhanced in comparison with the parental strain. SN - 0021-9193 UR - https://www.unboundmedicine.com/medline/citation/7430068/Role_and_control_of_isocitrate_lyase_in_Candida_lipolytica_ L2 - https://journals.asm.org/doi/10.1128/jb.144.2.692-697.1980?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -