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[New findings on Junin virus infection in rodents inside and outside the endemic area of hemorrhagic fever in Argentina].
Medicina (B Aires). 1991; 51(6):519-23.M

Abstract

In conjunction with field trials for a vaccine against Argentine Hemorrhagic Fever (AHF), small mammals were trapped during a 28-month period (1 November 1987 to 13 March 1990) in 3 epidemiologically defined areas of the central Argentine pampas: northern and central Buenos Aires provinces were included in the AHF "historic" area, where the disease was common 15-20 years ago, but case rates are currently low; southern Santa Fe province is the current high-incidence area for AHF; the nonendemic area was represented by two localities 60-90 km beyond the northernmost extension of human disease. Animals were live-trapped for 3 days per month in permanent "mark-recapture" grids in each of the 3 areas. Samples of blood, sera, and oral swabs were taken from these animals before they were marked and released at the site of capture. In addition, "removal" traplines provided animals from 16 localities in these 3 areas which were sacrificed to obtain samples of organs in addition to the aforementioned samples. Samples were tested for the presence of Junin virus (JV) antigen by enzyme immunoassay (ELISA). In this assay, a pool of 13 mouse anti-JV glycoprotein and nucleocapsid monoclonal antibodies adsorbed to the surface of microtiter plates was used to capture JV antigen in sample suspensions. A polyclonal rabbit anti-JV antiserum was added as a detector antibody, and an anti-rabbit antibody conjugated to horseradish peroxidase applied with substrate to complete the sandwich.(

ABSTRACT

TRUNCATED AT 250 WORDS)

Authors+Show Affiliations

Department of Immunology and Infectious Diseases, Johns Hopkins University School of Hygiene and Public Health, Baltimore, MD, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

spa

PubMed ID

7476104

Citation

Mills, J N., et al. "[New Findings On Junin Virus Infection in Rodents Inside and Outside the Endemic Area of Hemorrhagic Fever in Argentina]." Medicina, vol. 51, no. 6, 1991, pp. 519-23.
Mills JN, Calderón GE, Ellis BA, et al. [New findings on Junin virus infection in rodents inside and outside the endemic area of hemorrhagic fever in Argentina]. Medicina (B Aires). 1991;51(6):519-23.
Mills, J. N., Calderón, G. E., Ellis, B. A., McKee, K. T., Ksiazek, T. G., Oro, J. G., Peters, C. J., Childs, J. E., & Maiztegui, J. I. (1991). [New findings on Junin virus infection in rodents inside and outside the endemic area of hemorrhagic fever in Argentina]. Medicina, 51(6), 519-23.
Mills JN, et al. [New Findings On Junin Virus Infection in Rodents Inside and Outside the Endemic Area of Hemorrhagic Fever in Argentina]. Medicina (B Aires). 1991;51(6):519-23. PubMed PMID: 7476104.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [New findings on Junin virus infection in rodents inside and outside the endemic area of hemorrhagic fever in Argentina]. AU - Mills,J N, AU - Calderón,G E, AU - Ellis,B A, AU - McKee,K T, AU - Ksiazek,T G, AU - Oro,J G, AU - Peters,C J, AU - Childs,J E, AU - Maiztegui,J I, PY - 1991/1/1/pubmed PY - 1991/1/1/medline PY - 1991/1/1/entrez SP - 519 EP - 23 JF - Medicina JO - Medicina (B Aires) VL - 51 IS - 6 N2 - In conjunction with field trials for a vaccine against Argentine Hemorrhagic Fever (AHF), small mammals were trapped during a 28-month period (1 November 1987 to 13 March 1990) in 3 epidemiologically defined areas of the central Argentine pampas: northern and central Buenos Aires provinces were included in the AHF "historic" area, where the disease was common 15-20 years ago, but case rates are currently low; southern Santa Fe province is the current high-incidence area for AHF; the nonendemic area was represented by two localities 60-90 km beyond the northernmost extension of human disease. Animals were live-trapped for 3 days per month in permanent "mark-recapture" grids in each of the 3 areas. Samples of blood, sera, and oral swabs were taken from these animals before they were marked and released at the site of capture. In addition, "removal" traplines provided animals from 16 localities in these 3 areas which were sacrificed to obtain samples of organs in addition to the aforementioned samples. Samples were tested for the presence of Junin virus (JV) antigen by enzyme immunoassay (ELISA). In this assay, a pool of 13 mouse anti-JV glycoprotein and nucleocapsid monoclonal antibodies adsorbed to the surface of microtiter plates was used to capture JV antigen in sample suspensions. A polyclonal rabbit anti-JV antiserum was added as a detector antibody, and an anti-rabbit antibody conjugated to horseradish peroxidase applied with substrate to complete the sandwich.(ABSTRACT TRUNCATED AT 250 WORDS) SN - 0025-7680 UR - https://www.unboundmedicine.com/medline/citation/7476104/[New_findings_on_Junin_virus_infection_in_rodents_inside_and_outside_the_endemic_area_of_hemorrhagic_fever_in_Argentina]_ DB - PRIME DP - Unbound Medicine ER -