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Whole body arginine metabolism and nitric oxide synthesis in newborns with persistent pulmonary hypertension.
Pediatr Res. 1995 Jul; 38(1):17-24.PR

Abstract

Despite the potential relevance of the L-arginine-nitric oxide (NO) pathway in the pathophysiology of pulmonary hypertension, no in vivo studies of the kinetics of arginine and NO have been conducted previously in this population. The terminal guanidino N-atom of L-arginine is the precursor for NO, which is oxidized to the stable inorganic nitrogen oxides, nitrite (NO2-) and nitrate (NO3-). Thus, synthesized NO is detected in serum or urine as NO2- and NO3-. The purpose of this investigation was to compare studies of whole body arginine metabolism twice in nine patients with persistent pulmonary hypertension of the newborn (PPHN), using a primed constant i.v. infusion of L-[guanidino-15N2,5,5(2)H2]arginine and L-[5,5,5(2)H3]leucine, first during acute pulmonary vasoconstriction and again during convalescence, and thereby to characterize quantitative aspects of whole body arginine kinetics and NO production, as estimated from the rate of transfer of the 15N-guanidino-label of arginine to urinary nitrate (15NO3-). Arginine flux rates were 84.1 +/- 8.6 mumol.kg-1 h-1 (mean +/- SEM) during acute pulmonary hypertension and increased to 125 +/- 13.2 (p < 0.05) during convalescence, whereas leucine fluxes were unchanged (168.5 +/- 15 versus 178.8 +/- 10.2 mumol.kg-1 h-1), and comparable to those reported in healthy newborns. During convalescence total urinary nitrate excreted increased by 66% (p < 0.05), urinary 15NO3- increased from 0.29 +/- 0.07 to 0.74 +/- 0.15 mumol.d-1 (p < 0.05), and the rate of plasma arginine conversion to NO increased from 10.3 +/- 2.2 to 45.6 +/- 13 mumol.d-1 (p < 0.05).(

ABSTRACT

TRUNCATED AT 250 WORDS)

Authors+Show Affiliations

Laboratory of Human Nutrition, Massachusetts Institute of Technology, Boston 02139, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

7478791

Citation

Castillo, L, et al. "Whole Body Arginine Metabolism and Nitric Oxide Synthesis in Newborns With Persistent Pulmonary Hypertension." Pediatric Research, vol. 38, no. 1, 1995, pp. 17-24.
Castillo L, DeRojas-Walker T, Yu YM, et al. Whole body arginine metabolism and nitric oxide synthesis in newborns with persistent pulmonary hypertension. Pediatr Res. 1995;38(1):17-24.
Castillo, L., DeRojas-Walker, T., Yu, Y. M., Sanchez, M., Chapman, T. E., Shannon, D., Tannenbaum, S., Burke, J. F., & Young, V. R. (1995). Whole body arginine metabolism and nitric oxide synthesis in newborns with persistent pulmonary hypertension. Pediatric Research, 38(1), 17-24.
Castillo L, et al. Whole Body Arginine Metabolism and Nitric Oxide Synthesis in Newborns With Persistent Pulmonary Hypertension. Pediatr Res. 1995;38(1):17-24. PubMed PMID: 7478791.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Whole body arginine metabolism and nitric oxide synthesis in newborns with persistent pulmonary hypertension. AU - Castillo,L, AU - DeRojas-Walker,T, AU - Yu,Y M, AU - Sanchez,M, AU - Chapman,T E, AU - Shannon,D, AU - Tannenbaum,S, AU - Burke,J F, AU - Young,V R, PY - 1995/7/1/pubmed PY - 1995/7/1/medline PY - 1995/7/1/entrez SP - 17 EP - 24 JF - Pediatric research JO - Pediatr. Res. VL - 38 IS - 1 N2 - Despite the potential relevance of the L-arginine-nitric oxide (NO) pathway in the pathophysiology of pulmonary hypertension, no in vivo studies of the kinetics of arginine and NO have been conducted previously in this population. The terminal guanidino N-atom of L-arginine is the precursor for NO, which is oxidized to the stable inorganic nitrogen oxides, nitrite (NO2-) and nitrate (NO3-). Thus, synthesized NO is detected in serum or urine as NO2- and NO3-. The purpose of this investigation was to compare studies of whole body arginine metabolism twice in nine patients with persistent pulmonary hypertension of the newborn (PPHN), using a primed constant i.v. infusion of L-[guanidino-15N2,5,5(2)H2]arginine and L-[5,5,5(2)H3]leucine, first during acute pulmonary vasoconstriction and again during convalescence, and thereby to characterize quantitative aspects of whole body arginine kinetics and NO production, as estimated from the rate of transfer of the 15N-guanidino-label of arginine to urinary nitrate (15NO3-). Arginine flux rates were 84.1 +/- 8.6 mumol.kg-1 h-1 (mean +/- SEM) during acute pulmonary hypertension and increased to 125 +/- 13.2 (p < 0.05) during convalescence, whereas leucine fluxes were unchanged (168.5 +/- 15 versus 178.8 +/- 10.2 mumol.kg-1 h-1), and comparable to those reported in healthy newborns. During convalescence total urinary nitrate excreted increased by 66% (p < 0.05), urinary 15NO3- increased from 0.29 +/- 0.07 to 0.74 +/- 0.15 mumol.d-1 (p < 0.05), and the rate of plasma arginine conversion to NO increased from 10.3 +/- 2.2 to 45.6 +/- 13 mumol.d-1 (p < 0.05).(ABSTRACT TRUNCATED AT 250 WORDS) SN - 0031-3998 UR - https://www.unboundmedicine.com/medline/citation/7478791/Whole_body_arginine_metabolism_and_nitric_oxide_synthesis_in_newborns_with_persistent_pulmonary_hypertension_ L2 - http://dx.doi.org/10.1203/00006450-199507000-00004 DB - PRIME DP - Unbound Medicine ER -