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Immunology and morphology studies on the proliferation of in vitro cultivated Echinococcus multilocularis metacestodes.
Parasitol Res 1995; 81(7):605-14PR

Abstract

The larval stage of Echinococcus multilocularis causes alveolar echinococcosis (AE) in various mammals, including humans. Traditionally metacestodes are maintained in the laboratory by serial transplantation passages into susceptible animals such as mice or gerbils. However, in animal models it has always been difficult to draw definite conclusions about the factors modulating metacestode differentiation, and investigations on gene expression and respective regulation have been hampered by the complexicity of the host-parasite interplay. This paper describes the maintenance and proliferation of E. multilocularis metacestodes as well as the formation of protoscolices in a chemically defined medium devoid of host influence. The interactive role of a heterologous human cell line (CACO2) in the in vitro development of metacestodes was also assessed. The morphology and ultrastructure of in vitro-generated metacestodes was studied using scanning (SEM) and transmission electron microscopy (TEM). Different cultivation procedures were analyzed in terms of expression of B- and T-cell epitopes and of the relevant laminated layer-antigen Em2; the exact localization of this antigen was further demonstrated by immunogold electron microscopy.

Authors+Show Affiliations

Institute of Parasitology, Faculty of Veterinary Medicine, University of Berne, Switzerland.No affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

7479653

Citation

Hemphill, A, and B Gottstein. "Immunology and Morphology Studies On the Proliferation of in Vitro Cultivated Echinococcus Multilocularis Metacestodes." Parasitology Research, vol. 81, no. 7, 1995, pp. 605-14.
Hemphill A, Gottstein B. Immunology and morphology studies on the proliferation of in vitro cultivated Echinococcus multilocularis metacestodes. Parasitol Res. 1995;81(7):605-14.
Hemphill, A., & Gottstein, B. (1995). Immunology and morphology studies on the proliferation of in vitro cultivated Echinococcus multilocularis metacestodes. Parasitology Research, 81(7), pp. 605-14.
Hemphill A, Gottstein B. Immunology and Morphology Studies On the Proliferation of in Vitro Cultivated Echinococcus Multilocularis Metacestodes. Parasitol Res. 1995;81(7):605-14. PubMed PMID: 7479653.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Immunology and morphology studies on the proliferation of in vitro cultivated Echinococcus multilocularis metacestodes. AU - Hemphill,A, AU - Gottstein,B, PY - 1995/1/1/pubmed PY - 1995/1/1/medline PY - 1995/1/1/entrez SP - 605 EP - 14 JF - Parasitology research JO - Parasitol. Res. VL - 81 IS - 7 N2 - The larval stage of Echinococcus multilocularis causes alveolar echinococcosis (AE) in various mammals, including humans. Traditionally metacestodes are maintained in the laboratory by serial transplantation passages into susceptible animals such as mice or gerbils. However, in animal models it has always been difficult to draw definite conclusions about the factors modulating metacestode differentiation, and investigations on gene expression and respective regulation have been hampered by the complexicity of the host-parasite interplay. This paper describes the maintenance and proliferation of E. multilocularis metacestodes as well as the formation of protoscolices in a chemically defined medium devoid of host influence. The interactive role of a heterologous human cell line (CACO2) in the in vitro development of metacestodes was also assessed. The morphology and ultrastructure of in vitro-generated metacestodes was studied using scanning (SEM) and transmission electron microscopy (TEM). Different cultivation procedures were analyzed in terms of expression of B- and T-cell epitopes and of the relevant laminated layer-antigen Em2; the exact localization of this antigen was further demonstrated by immunogold electron microscopy. SN - 0932-0113 UR - https://www.unboundmedicine.com/medline/citation/7479653/Immunology_and_morphology_studies_on_the_proliferation_of_in_vitro_cultivated_Echinococcus_multilocularis_metacestodes_ L2 - https://boris.unibe.ch/id/eprint/118688 DB - PRIME DP - Unbound Medicine ER -