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Detection of microsporidia (Enterocytozoon bieneusi) in intestinal biopsy specimens from human immunodeficiency virus-infected patients by PCR.
J Clin Microbiol. 1995 Sep; 33(9):2294-6.JC

Abstract

Intestinal microsporidiosis has been implicated as a major cause of chronic diarrhea in human immunodeficiency virus (HIV)-infected patients. So far diagnosis depends on direct visualization of the parasites by light and transmission electron microscopy. We evaluated the diagnostic value of microsporidian DNA amplification by PCR on duodenal biopsy specimens obtained from patients with and without intestinal microsporidiosis caused by Enterocytozoon bieneusi. Thirteen HIV-infected patients (all CDC stage C3) were studied. Eight patients had intestinal microsporidiosis caused by E. bieneusi (n = 6), Septata intestinalis (n = 1), and Encephalitozoon cuniculi (n = 1); microsporidioses were diagnosed by light microscopy of stool samples and confirmed by light and electron microscopy of intestinal biopsy specimens. Five patients had no microsporidia in their stool samples or in their intestinal biopsy specimens, as examined by light and electron microscopy. Additionally, DNA prepared from Toxoplasma gondii derived from mouse ascites was used as a further control. A 353-bp DNA fragment of the small-subunit rRNA gene could be amplified from all six biopsy specimens infected with E. bieneusi, and the nature of the PCR products was confirmed by Southern blot hybridization. No amplification of DNA fragments was seen by using DNA extracted from biopsy specimens with S. intestinalis or E. cuniculi infection or without microsporidian infection and with template DNA extracted from T. gondii. The results suggest that PCR testing of intestinal biopsy specimens may be a useful approach to diagnosing microsporidiosis in HIV-infected patients.

Authors+Show Affiliations

Department of Internal Medicine I, University of Cologne, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

7494017

Citation

Franzen, C, et al. "Detection of Microsporidia (Enterocytozoon Bieneusi) in Intestinal Biopsy Specimens From Human Immunodeficiency Virus-infected Patients By PCR." Journal of Clinical Microbiology, vol. 33, no. 9, 1995, pp. 2294-6.
Franzen C, Müller A, Hegener P, et al. Detection of microsporidia (Enterocytozoon bieneusi) in intestinal biopsy specimens from human immunodeficiency virus-infected patients by PCR. J Clin Microbiol. 1995;33(9):2294-6.
Franzen, C., Müller, A., Hegener, P., Salzberger, B., Hartmann, P., Fätkenheuer, G., Diehl, V., & Schrappe, M. (1995). Detection of microsporidia (Enterocytozoon bieneusi) in intestinal biopsy specimens from human immunodeficiency virus-infected patients by PCR. Journal of Clinical Microbiology, 33(9), 2294-6.
Franzen C, et al. Detection of Microsporidia (Enterocytozoon Bieneusi) in Intestinal Biopsy Specimens From Human Immunodeficiency Virus-infected Patients By PCR. J Clin Microbiol. 1995;33(9):2294-6. PubMed PMID: 7494017.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of microsporidia (Enterocytozoon bieneusi) in intestinal biopsy specimens from human immunodeficiency virus-infected patients by PCR. AU - Franzen,C, AU - Müller,A, AU - Hegener,P, AU - Salzberger,B, AU - Hartmann,P, AU - Fätkenheuer,G, AU - Diehl,V, AU - Schrappe,M, PY - 1995/9/1/pubmed PY - 1995/9/1/medline PY - 1995/9/1/entrez SP - 2294 EP - 6 JF - Journal of clinical microbiology JO - J Clin Microbiol VL - 33 IS - 9 N2 - Intestinal microsporidiosis has been implicated as a major cause of chronic diarrhea in human immunodeficiency virus (HIV)-infected patients. So far diagnosis depends on direct visualization of the parasites by light and transmission electron microscopy. We evaluated the diagnostic value of microsporidian DNA amplification by PCR on duodenal biopsy specimens obtained from patients with and without intestinal microsporidiosis caused by Enterocytozoon bieneusi. Thirteen HIV-infected patients (all CDC stage C3) were studied. Eight patients had intestinal microsporidiosis caused by E. bieneusi (n = 6), Septata intestinalis (n = 1), and Encephalitozoon cuniculi (n = 1); microsporidioses were diagnosed by light microscopy of stool samples and confirmed by light and electron microscopy of intestinal biopsy specimens. Five patients had no microsporidia in their stool samples or in their intestinal biopsy specimens, as examined by light and electron microscopy. Additionally, DNA prepared from Toxoplasma gondii derived from mouse ascites was used as a further control. A 353-bp DNA fragment of the small-subunit rRNA gene could be amplified from all six biopsy specimens infected with E. bieneusi, and the nature of the PCR products was confirmed by Southern blot hybridization. No amplification of DNA fragments was seen by using DNA extracted from biopsy specimens with S. intestinalis or E. cuniculi infection or without microsporidian infection and with template DNA extracted from T. gondii. The results suggest that PCR testing of intestinal biopsy specimens may be a useful approach to diagnosing microsporidiosis in HIV-infected patients. SN - 0095-1137 UR - https://www.unboundmedicine.com/medline/citation/7494017/Detection_of_microsporidia__Enterocytozoon_bieneusi__in_intestinal_biopsy_specimens_from_human_immunodeficiency_virus_infected_patients_by_PCR_ L2 - https://journals.asm.org/doi/10.1128/jcm.33.9.2294-2296.1995?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -