TY - JOUR T1 - BAP2, a gene encoding a permease for branched-chain amino acids in Saccharomyces cerevisiae. AU - Grauslund,M, AU - Didion,T, AU - Kielland-Brandt,M C, AU - Andersen,H A, PY - 1995/11/30/pubmed PY - 1995/11/30/medline PY - 1995/11/30/entrez SP - 275 EP - 80 JF - Biochimica et biophysica acta JO - Biochim Biophys Acta VL - 1269 IS - 3 N2 - To select the gene coding for an isoleucine permease, an isoleucine dependent strain (ilv1 cha1) was transformed with a yeast genomic multicopy library, and colonies growing at a low isoleucine concentration were selected. Partial sequencing of the responsible plasmid insert revealed the presence of a previously sequenced 609 codon open reading frame of chromosome II with homology to known permeases. Deletion, extra dosage and C-terminal truncation of this gene were constructed in a strain lacking the general amino acid permease, and amino acid uptake was measured during growth in synthetic complete medium. The following observations prompted us to name the gene BAP2 (branched-chain amino acid permease). Deletion of BAP2 reduced uptake of leucine, isoleucine and valine by 25-50%, while the uptake of 8 other L-alpha-amino acids was unaltered or slightly increased. Introduction of BAP2 on a centromere-based vector, leading to a gene dosage of two or slightly more, caused a 50% increase in leucine uptake and a smaller increase for isoleucine and valine. However, when the 29 C-terminal codons of the plasmid-borne copy of BAP2 were substituted, the cells more than doubled the uptake of leucine, isoleucine and valine, while no or little increase in uptake was observed for the other 8 amino acids. SN - 0006-3002 UR - https://www.unboundmedicine.com/medline/citation/7495881/BAP2_a_gene_encoding_a_permease_for_branched_chain_amino_acids_in_Saccharomyces_cerevisiae_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0167-4889(95)00138-8 DB - PRIME DP - Unbound Medicine ER -