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The modulation of the oxidative phase of the pentose phosphate pathway in mouse liver.
Int J Biochem Cell Biol. 1995 Oct; 27(10):1015-9.IJ

Abstract

The glucose-6-phosphate dehydrogenase from mouse liver is fully inhibited in vitro by physiological concentrations of NADPH. This suggests that the oxidative phase of the pentose phosphate pathway requires some deinhibitory system. In order to investigate regulation of the pentose phosphate pathway, various parameters (intermediate concentrations, mass-action ratios of reactions, etc.) were measured in liver from control mice and from meal-fed mice. Assays were also carried out to detect any molecules causing the reverse of glucose-6-phosphate dehydrogenase inhibition by NADPH. The liver of meal-fed mice show greater glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase activities. They also had greater concentrations of several metabolic intermediates and triglycerides than the control animals (P < 0.001). These results prove that the diet increases the flow of the pentose phosphate pathway in a lipogenic sense. The glutathione reductase does not change with the diet, suggesting that this enzyme does not participate in the modulating process. Unlike rat liver, no molecules causing the reverse of glucose-6-phosphate dehydrogenase inhibition by NADPH were detected. These data suggest that the increase of flow of the pentose phosphate pathway during lipogenesis is obtained by an increase in enzyme synthesis.

Authors+Show Affiliations

Departamento de Bioquímica y Biología Molecular, Universidad de Santiago de Compostela, Facultad de Veterinaria, Lugo, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

7496990

Citation

Velasco, P, et al. "The Modulation of the Oxidative Phase of the Pentose Phosphate Pathway in Mouse Liver." The International Journal of Biochemistry & Cell Biology, vol. 27, no. 10, 1995, pp. 1015-9.
Velasco P, Sieiro AM, Ibarguren I, et al. The modulation of the oxidative phase of the pentose phosphate pathway in mouse liver. Int J Biochem Cell Biol. 1995;27(10):1015-9.
Velasco, P., Sieiro, A. M., Ibarguren, I., Ramos-Martínez, J. I., & Barcia, R. (1995). The modulation of the oxidative phase of the pentose phosphate pathway in mouse liver. The International Journal of Biochemistry & Cell Biology, 27(10), 1015-9.
Velasco P, et al. The Modulation of the Oxidative Phase of the Pentose Phosphate Pathway in Mouse Liver. Int J Biochem Cell Biol. 1995;27(10):1015-9. PubMed PMID: 7496990.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The modulation of the oxidative phase of the pentose phosphate pathway in mouse liver. AU - Velasco,P, AU - Sieiro,A M, AU - Ibarguren,I, AU - Ramos-Martínez,J I, AU - Barcia,R, PY - 1995/10/1/pubmed PY - 1995/10/1/medline PY - 1995/10/1/entrez SP - 1015 EP - 9 JF - The international journal of biochemistry & cell biology JO - Int. J. Biochem. Cell Biol. VL - 27 IS - 10 N2 - The glucose-6-phosphate dehydrogenase from mouse liver is fully inhibited in vitro by physiological concentrations of NADPH. This suggests that the oxidative phase of the pentose phosphate pathway requires some deinhibitory system. In order to investigate regulation of the pentose phosphate pathway, various parameters (intermediate concentrations, mass-action ratios of reactions, etc.) were measured in liver from control mice and from meal-fed mice. Assays were also carried out to detect any molecules causing the reverse of glucose-6-phosphate dehydrogenase inhibition by NADPH. The liver of meal-fed mice show greater glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase activities. They also had greater concentrations of several metabolic intermediates and triglycerides than the control animals (P < 0.001). These results prove that the diet increases the flow of the pentose phosphate pathway in a lipogenic sense. The glutathione reductase does not change with the diet, suggesting that this enzyme does not participate in the modulating process. Unlike rat liver, no molecules causing the reverse of glucose-6-phosphate dehydrogenase inhibition by NADPH were detected. These data suggest that the increase of flow of the pentose phosphate pathway during lipogenesis is obtained by an increase in enzyme synthesis. SN - 1357-2725 UR - https://www.unboundmedicine.com/medline/citation/7496990/The_modulation_of_the_oxidative_phase_of_the_pentose_phosphate_pathway_in_mouse_liver_ L2 - https://linkinghub.elsevier.com/retrieve/pii/1357272595000795 DB - PRIME DP - Unbound Medicine ER -